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Novel phosphorylation substrates reveal a spatially regulated role for AAK1 in cell migration - AAK1 differential interactome profiling

ABSTRACT: NAKs (Numb-associated kinases) are a relatively unexplored kinase family that interacts with the endocytic machinery. Among NAK members, AAK1 has been linked to disorders such as neuropathic pain, schizophrenia, and Alzheimer's disease. Our goal is to better understand the cellular pathways regulated by AAK1 as its only known role until now has been in endocytosis. To define the AAK1 interactome in relation to focal adhesions, we compared its interactome in cells plated for 2 hours on collagen, which promotes focal adhesions, and poly-L-lysine (PLL), which does not. In collagen-plated cells, we discovered several differential AAK1 interactors involved in focal adhesions, the actin cytoskeleton, and recycling endosomes. In PLL-plated cells, there was a significant increase in actin cytoskeleton components and upregulation of the entire Arp2/3 complex as AAK1 interactors. This supports our other data showing that AAK1 is involved in focal adhesion maintenance and cell migration.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: David Potesil

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD055873 | Pride | 2026-03-19

REPOSITORIES: Pride

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			Action	DRS
	5816_TIMS1_RSLC-74m_TFA_DIA_10_01_BB2_1_28668.d.zip	Other
	5816_TIMS1_RSLC-74m_TFA_DIA_11_01_BB3_1_28652.d.zip	Other
	5816_TIMS1_RSLC-74m_TFA_DIA_12_01_BB4_1_28777.d.zip	Other
	5816_TIMS1_RSLC-74m_TFA_DIA_1_01_BA1_1_28761.d.zip	Other
	5816_TIMS1_RSLC-74m_TFA_DIA_2_01_BA2_1_28697.d.zip	Other

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