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Shotgun proteomics analysis of meagre (Argyrosomus regius) blood plasma and skin mucus

ABSTRACT: In aquaculture, meagre (Argyrosomus regius), it still produced on a small scale, nevertheless it is a species with great potential to expand due to its beneficial traits both for production and consumption. The development of new functional diets for this species that can promote both growth and health is an important step towards the increase of its production. Studying fish health and nutrition status can be complex and cause additional stress to the fish, and often require animal sacrifice. Therefore, it becomes important to find proper techniques and biomarkers that allow a better measurement of the effects of new diets and at the same time are practical to apply in an aquaculture setting. Using non-lethal measurements to evaluate fish health and welfare has generated interest as it allows for less animal sacrifice and reduced production losses. This type of analysis includes parameters from samples of blood, mucus, skin and faeces, which are important tools for monitoring fish health and welfare. In this study, a nutritional challenge was performed for 7 weeks with juvenile meagre, where three diets were tested, a control diet (55.1 % of fish meal, FM, control), a challenging diet (15% FM, challenge) and an extremely challenging diet (5% FM, extreme). The main objective was to identify candidate minimally invasive biomarkers of malnutrition in blood plasma and skin mucus.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Argyrosomus Regius

TISSUE(S): Blood Plasma, Skin Mucus

SUBMITTER: Cláudia Raposo de Magalhães

LAB HEAD: Ana Couto

PROVIDER: PXD055888 | Pride | 2025-05-29

REPOSITORIES: Pride

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			Action	DRS
	2023-06-29-decoys-contam-uniprot_eupercaria_895538_2023-06-27.fasta	Fasta
	M_T1_P2.pepXML	Pepxml
	M_T1_P2.raw	Raw
	M_T1_P5.pepXML	Pepxml
	M_T1_P5.raw	Raw

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