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Ethanol Exposure during Differentiation of Human Induced Pluripotent Stem Cells Reduces Cardiomyocyte Generation and Alters Metabolism

ABSTRACT: In this study, we investigated the effects of ethanol exposure on cardiomyocyte differentiation by treating the cells with various concentrations of ethanol. The results indicated that ethanol exposure during hiPSC-CM differentiation reduced cell viability, cell proliferation, and cardiomyocyte yield, and caused mitochondrial dysfunction, redox imbalance, and changes of proteomic profiles.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Longping Fu

LAB HEAD: Ronghu Wu

PROVIDER: PXD056092 | Pride | 2025-05-23

REPOSITORIES: Pride

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	E_LF_Kun_Day12_10_04242024.pepXML	Pepxml
	E_LF_Kun_Day12_10_04242024.raw	Raw
	E_LF_Kun_Day12_11_04242024.pepXML	Pepxml
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Ethanol exposure during differentiation of human induced pluripotent stem cells reduces cardiomyocyte generation and alters metabolism.

Man Kun K Fu Longping L Lane Alicia A Harris Frank F Reid Olivia O Armand Lawrence C LC Forghani Parvin P Wu Ronghu R Faundez Victor V Brown Lou Ann LA Xu Chunhui C

Life sciences 20250130

Prenatal alcohol exposure increases the risk of congenital heart diseases (CHDs) by disrupting fetal development, yet the mechanisms underlying alcohol-induced cellular and molecular changes in human cardiogenesis remain unclear. This study investigates the effects of ethanol exposure on cardiomyocyte differentiation using human induced pluripotent stem cells (hiPSCs) as a model. Cardiomyocyte differentiation was induced using Wnt signaling molecules, and hiPSCs were treated with ethanol at conc ...[more]

PMID: 39892862

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