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Mouse embryonic stem cells heterochromatin-associated protein and Nat10 IP-MS

ABSTRACT: The sonication-resistant heterochromatin (srHC) fragments of mESCs were isolated by sucrose gradient ultracentrifugation, and the heterochromatin-associated proteins were enriched by Co-IP combined with mass spectrometry (Co-IP/MS) using anti-H3K9me3, IgG was used as a negative control. The Nat10 interacted proteins in mESCs were isolated by 3×Flag-Nat10 fusion protein overexpression and performed IP-MS using anti-Flag, IgG antibody was used as a negative control.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Guoxing Yin

LAB HEAD: Guofeng Feng

PROVIDER: PXD056153 | Pride | 2025-06-23

REPOSITORIES: Pride

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			Action	DRS
	FA347GI_IgG.raw	Raw
	FA347GI_IgG_Mass.xlsx	Xlsx
	FA347GI_MIX.raw	Raw
	FA347GI_MIX_Mass.xlsx	Xlsx
	SD081GI_FLAG_IP.raw	Raw

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Publications

Nat10 maintains the homeostasis of pluripotent and 2-cell-like states in mouse embryonic stem cells through mRNA cytidine acetylation.

Feng Guofeng G Yin Guoxing G Liu Yusheng Y Liu Chang C Li Jie J Lu Jiangtao J Yu Yongqin Y Jin Ziyi Z Wu Yiwei Y Yue Yanbin Y Gao Xiufang X Wang Jiaqiang J Lu Falong F Liu Lin L

Nucleic acids research 20250601 11

Naïve mouse embryonic stem cells (mESCs) are characterized by a mixed population of cells in an interconvertible pluripotent state and a totipotent 2-cell (2C)-like state. It remains to be understood how the pluripotent state is maintained while the 2C-like state is suppressed. We show that N-acetyltransferase 10 (Nat10) maintains the pluripotent state and suppresses the 2C-like state in mESCs through mRNA modification and stabilization. Nat10 as a nucleolar protein may indirectly interact with ...[more]

PMID: 40521666

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