Mouse embryonic stem cells heterochromatin-associated protein and Nat10 IP-MS

ABSTRACT: The sonication-resistant heterochromatin (srHC) fragments of mESCs were isolated by sucrose gradient ultracentrifugation, and the heterochromatin-associated proteins were enriched by Co-IP combined with mass spectrometry (Co-IP/MS) using anti-H3K9me3, IgG was used as a negative control. The Nat10 interacted proteins in mESCs were isolated by 3×Flag-Nat10 fusion protein overexpression and performed IP-MS using anti-Flag, IgG antibody was used as a negative control.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Guoxing Yin  

LAB HEAD: Guofeng Feng

PROVIDER: PXD056153 | Pride | 2025-06-23

REPOSITORIES: Pride