Dataset Information

Identification of Estrogen-Responsive Proteins in the Mouse Seminal Vesicle by Mass Spectrometry-Based Proteomics

ABSTRACT: Although estrogenic compounds promise therapeutic potential in treating various conditions, concerns regarding their endocrine-disrupting effects have been raised Current methodologies for screening estrogenicity in rodent models are limited to the female-specific uterotrophic bioassay. Studies have reported enlargement of seminal vesicles in orchiectomized males treated with estrogens. However, identifying estrogenicity strictly through changes in wet weights is uninformative regarding the molecular mechanisms of these agents. Therefore, protein-based biomarkers can complement and improve the sensitivity of the weight-based assessments. To this end, we present a discovery-driven proteomic analysis on 17β-estradiol's effects on the seminal vesicles. We treated orchidectomized mice with the hormone for five days, and used the vehicle-treated group as control. Differentially expressed proteins in the seminal vesicles between the two groups were identified through label-free quantification using data-dependent nanoflow liquid chromatography–tandem mass spectrometry. We identified 668 total proteins, of which 133 were found to be regulated significantly by 17β-estradiol. Ingenuity Pathway Analysis® linked them to several hormone-affected pathways, including those associated with immune function such as neutrophil degranulation. The altered protein interaction networks were also related to functions, including endocrine disruption, abnormal metabolism, and therapeutic effects. Overall, we identified several potential biomarkers for estrogenicity in the mouse seminal vesicles including those not previously linked with exogenous 17β-estradiol exposure such as spondin-1

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Seminal Vesicle

SUBMITTER: Laszlo Prokai

LAB HEAD: Laszlo Prokai, Ph.D., D.Sc

PROVIDER: PXD056329 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	09082020_SV_C1_1__09082020_SV_C1_1.mzid.gz	Mzid
	09082020_SV_C1_1__09082020_SV_C1_1.mzid_09082020_SV_C1_1__09082020_SV_C1_1.MGF	Mzid
	09082020_SV_C1_1__09082020_SV_C1_2.mzid.gz	Mzid
	09082020_SV_C1_1__09082020_SV_C1_2.mzid_09082020_SV_C1_1__09082020_SV_C1_2.MGF	Mzid
	09082020_SV_C1_1__09082020_SV_C2_1.mzid.gz	Mzid

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Identification of Estrogen-Responsive Proteins in Mouse Seminal Vesicles Through Mass Spectrometry-Based Proteomics.

Kapic Ammar A Zaman Khadiza K Nguyen Vien V Prokai-Tatrai Katalin K Prokai Laszlo L

Pharmaceuticals (Basel, Switzerland) 20241109 11

<b>Background:</b> Although estrogenic compounds promise therapeutic potential in treating various conditions, concerns regarding their endocrine-disrupting effects have been raised. Current methodologies for screening estrogenicity in rodent models are limited to the female-specific uterotrophic bioassay. Studies have reported enlargement of the seminal vesicles in orchiectomized males treated with estrogens. However, identifying estrogenicity strictly through changes in wet weights is uninform ...[more]

PMID: 39598420

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