Dataset Information

Identification of Estrogen-Responsive Proteins in the Mouse Seminal Vesicle by Mass Spectrometry-Based Proteomics

ABSTRACT: Although estrogenic compounds promise therapeutic potential in treating various conditions, concerns regarding their endocrine-disrupting effects have been raised Current methodologies for screening estrogenicity in rodent models are limited to the female-specific uterotrophic bioassay. Studies have reported enlargement of seminal vesicles in orchiectomized males treated with estrogens. However, identifying estrogenicity strictly through changes in wet weights is uninformative regarding the molecular mechanisms of these agents. Therefore, protein-based biomarkers can complement and improve the sensitivity of the weight-based assessments. To this end, we present a discovery-driven proteomic analysis on 17β-estradiol's effects on the seminal vesicles. We treated orchidectomized mice with the hormone for five days, and used the vehicle-treated group as control. Differentially expressed proteins in the seminal vesicles between the two groups were identified through label-free quantification using data-dependent nanoflow liquid chromatography–tandem mass spectrometry. We identified 668 total proteins, of which 133 were found to be regulated significantly by 17β-estradiol. Ingenuity Pathway Analysis® linked them to several hormone-affected pathways, including those associated with immune function such as neutrophil degranulation. The altered protein interaction networks were also related to functions, including endocrine disruption, abnormal metabolism, and therapeutic effects. Overall, we identified several potential biomarkers for estrogenicity in the mouse seminal vesicles including those not previously linked with exogenous 17β-estradiol exposure such as spondin-1

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Seminal Vesicle

SUBMITTER: Laszlo Prokai

LAB HEAD: Laszlo Prokai, Ph.D., D.Sc

PROVIDER: PXD056329 | Pride | 2025-05-07

REPOSITORIES: pride

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			Action	DRS
	09082020_SV_C1_1__09082020_SV_C1_1.mzid.gz	Mzid
	09082020_SV_C1_1__09082020_SV_C1_1.mzid_09082020_SV_C1_1__09082020_SV_C1_1.MGF	Mzid
	09082020_SV_C1_1__09082020_SV_C1_2.mzid.gz	Mzid
	09082020_SV_C1_1__09082020_SV_C1_2.mzid_09082020_SV_C1_1__09082020_SV_C1_2.MGF	Mzid
	09082020_SV_C1_1__09082020_SV_C2_1.mzid.gz	Mzid

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Identification of Estrogen-Responsive Proteins in Mouse Seminal Vesicles Through Mass Spectrometry-Based Proteomics.

Kapic Ammar A Zaman Khadiza K Nguyen Vien V Prokai-Tatrai Katalin K Prokai Laszlo L

Pharmaceuticals (Basel, Switzerland) 20241109 11

<b>Background:</b> Although estrogenic compounds promise therapeutic potential in treating various conditions, concerns regarding their endocrine-disrupting effects have been raised. Current methodologies for screening estrogenicity in rodent models are limited to the female-specific uterotrophic bioassay. Studies have reported enlargement of the seminal vesicles in orchiectomized males treated with estrogens. However, identifying estrogenicity strictly through changes in wet weights is uninform ...[more]

PMID: 39598420

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Project description:Transcriptional profiling of mussel (Mytilus galloprovincialis) digestive gland tissue comparing control tissue with tissue obtained from animals exposed to sublethal amounts of Chlorpyrifos, animals injected into the posterior adductor muscle with 25 pico-moles 17β-estradiol (E2) and animals pre-exposed for three days to the pesticide and further injected with E2. Background: Many pesticides have been shown to act as endocrine disrupters. Although the potencies of currently used pesticides as hormone agonists/antagonists in vitro are low compared with those of natural ligands, their ability to act via multiple mechanisms might enhance the biological effect. The organophosphate Chlorpyrifos (CHP) has been shown to be weakly estrogenic and cause adverse neurodevelopmental effects in mammals. However, no information is available on the possible endocrine effects of CHP in aquatic organisms. In the digestive gland of the bivalve Mytilus galloprovincialis, a target tissue for the action of both estrogens and pesticides, the possible effects of CHP on the responses to the natural estrogen 17β-estradiol (E2) were investigated. Methodology/Principal findings: Mussels were exposed to CHP (4.5 mg/l, 72 hrs) and subsequently injected with E2 (6.75 ng/g dw). Responses were evaluated in CHP, E2 and CHP/E2 treatment groups at 24 h p.i. by a biomarker/transcriptomic approach. CHP and E2 induced additive, synergistic, and antagonistic effects on lysosomal biomarkers (lysosomal membrane stability, lysosome/cytoplasm volume ratio, lipofuscin and neutral lipid accumulation). Additive and synergistic effects were also observed on the expression of estrogen-responsive genes (GSTπ, catalase and 5-HTR) evaluated by RT-Q-PCR. The use of a 1.7K cDNA Mytilus microarray showed that CHP, E2 and CHP/E2, induced 81, 44, and 65 Differentially Expressed Genes (DEGs), respectively. 24 genes were exclusively shared between CHP and CHP/E2, only 2 genes between E2 and CHP/E2. Moreover, 36 genes were uniquely modulated by CHP/E2. Gene ontology annotation was used to elucidate the putative mechanisms involved in the responses elicited by different treatments. Conclusions: The results show complex interactions between CHP and E2 in mussel digestive gland, indicating that the combination of certain pesticides and hormones may give rise to unexpected effects at the molecular/cellular level. Overall, these data demonstrate that CHP can interfere with the mussel responses to natural estrogens. Four-condition experiment. Dual color competitive hybridizations. Common reference (vehicle treated animals, 0.02% Dimethyl sulfoxide, injected with 50 μl of a solution of Artificial Sea Water containing 0.05 % ethanol); Pools of six animals. Biological replicates: 4 controls, 4 Chlorpyrifos, 4 17β-estradiol, 4 Chlorpyrifos-17β-stradiol. One replicate per array.