Serum Disease-specific IgG Fc Glycosylation as Potential Biomarkers for Nonproliferative and Proliferative Diabetic Retinopathy Using Mass Spectrometry

ABSTRACT: The patients were enrolled and categorized into three groups according to clinical diagnosis: non-diabetic retinopathy; nonproliferative diabetic retinopathy ; and proliferative diabetic retinopathy. Gel electrophoresis was performed to separate IgG from morning fasting blood samples and polyaniline magnetic nanomaterials (Fe3O4@PANI) were used to enrich IgG N-glycopeptides from tryptic digestion. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI ToF MS) was used to detect the IgG N-glycopeptides.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

SUBMITTER: Yishuang Mao  

LAB HEAD: Zhili Li

PROVIDER: PXD057612 | Pride | 2025-06-16

REPOSITORIES: Pride