Pulldown of Maspin-GST in Human Cells Treated or Untreated with Nocodazole

ABSTRACT: Human cells, either asynchronous or synchronized with nocodazole, were subjected to a pulldown assay using GST-maspin. Interacting proteins were eluted using 10 mM reduced glutathione, 150 mM NaCl, 1 mM DTT, and 50 mM Tris-HCl (pH 8.5).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Breast Cancer

SUBMITTER: Julia Cunha  

LAB HEAD: Julia P.C da Cunha, Nathalie Cella

PROVIDER: PXD057847 | Pride | 2025-09-01

REPOSITORIES: Pride