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Proteomic analysis of BEAS-2B lung epithelial cells after the introduction of the BRAF V600E mutation using CRISPR/Cas9

ABSTRACT: To mimic the naturally occurring BRAF V600E mutation in lung cancer, a BRAF V600E knock-in BEAS-2B cell model was established using CRISPR/Cas9. The proteomics analysis was carried out to evaluate the underlying changes at the proteome level. Proteomics analysis revealed significant changes in the proteins involved in the biological processes including epithelial-mesenchymal transition (EMT), extracellular matrix (ECM)-receptor interaction, cell adhesion, focal adhesion, and cell metabolism upon BRAF V600E mutation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Lingyun Dai

LAB HEAD: Lingyun Dai

PROVIDER: PXD058027 | Pride | 2025-04-22

REPOSITORIES: Pride

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			Action	DRS
	20230116_BEAS_2B_VE_1.raw	Raw
	20230116_BEAS_2B_VE_1.raw.quant	Raw
	20230116_BEAS_2B_VE_2.raw	Raw
	20230116_BEAS_2B_VE_2.raw.quant	Raw
	20230116_BEAS_2B_VE_3.raw	Raw

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