Dataset Information

Identification of in cellulo Uranyl-Binding Proteins in Arabidopsis Cells

ABSTRACT: Uranium (U) is a naturally occurring radionuclide. The redistribution of U in the environment is primarily driven by anthropogenic activities, including mining operations, the nuclear industry, military activities, and soil fertilization. Local accumulation of U can pose potential risks to ecosystems, agricultural systems, and ultimately human health. Although U is not essential for plants, yet they take it up from the soil and incorporate it into their biomass. This eventually enters the food chain, posing a significant health risk to humans. To identify proteins that could be cellular targets for U in planta, we used a metalloproteomic approach combining column chromatographic fractionation analyses with protein identification by high-resolution mass spectrometry (MS)-based shotgun proteomics.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD058046 | Pride | 2025-08-01

REPOSITORIES: Pride

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Publications

Identification of uranyl-binding proteins in Arabidopsis thaliana cells exposed to uranium: Insights from a metalloproteomic analysis and characterization of Glycine-Rich RNA-binding protein 7 (GRP7).

Revel Benoît H BH Favier Adrien A Martin-Laffon Jacqueline J Vallet Alicia A Przybyla-Toscano Jonathan J Brugière Sabine S Couté Yohann Y Diemer Hélène H Cianférani Sarah S Rabilloud Thierry T Bourguignon Jacques J Brutscher Bernhard B Ravanel Stéphane S Alban Claude C

Journal of hazardous materials 20250707

Uranium (U) is a naturally occurring radionuclide that poses chemotoxic threats to living organisms, including plants. Despite its environmental relevance, the molecular mechanisms underlying U toxicity in plant cells are not well understood. In this study, we used an integrative metalloproteomic strategy integrating chromatographic fractionation with high-resolution proteomics and inductively coupled plasma mass spectrometry to identify cellular protein targets of uranyl (U(VI)) in cultured Ara ...[more]

PMID: 40633352

Similar Datasets

Project description:Plant cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes involved in Cys biosynthesis and located in different subcellular compartments. These enzymes are made up of a complex variety of isoforms resulting in different subcellular Cys pools. To unravel the contribution of cytosolic Cys to plant metabolism, we characterized the knockout oas-a1.1 and osa-a1.2 mutants, deficient in the most abundant cytosolic OASTL isoform in Arabidposis thaliana. Total intracellular Cys and glutathione concentrations were reduced, and the glutathione redox state was shifted in favour of its oxidized form. Interestingly, the capability of the mutants to chelate heavy metals did not differ from that of the wild type, but the mutants have an enhanced sensitivity to Cd. With the aim of establishing the metabolic network most influenced by the cytosolic Cys pool, we used the ATH1 GeneChip for evaluation of differentially expressed genes in the oas-a1.1 mutant grown under non-stress conditions. The transcriptomic footprints of mutant plants had predicted functions associated with various physiological responses that are dependent on reactive oxygen species and suggested that the mutant was oxidatively stressed. To further elucidate the specific function(s) of the OAS-A1 isoform in the adaptation response to cadmium we extended the trasncriptome experiment to the wild type and oas-a1.1 mutant plants exposed to Cd. The comparison of transcriptomic profiles showed a higher proportion of genes with altered expression in the mutant than in the wild type, highlighting up-regulated genes identified as of the general oxidative stress response rather than metal-responsive genes. Wild type and oas-a1.1 mutant plants were grown hydroponically and, after a two-week acclimation period, the roots and shoots were harvested separately. Total RNA was then prepared and analyzed using the Affymetrix-Arabidopsis ATH1GeneChip array. Three biological replicates were performed for each sample. We made two different comparisons to classify the differently expressed genes in the mutant plant: oas-a1.1 roots versus wild-type roots and oas-a1.1 shoots versus wild-type shoots. Hydroponically-grown wild type and oas-a1.1 mutant plants were further treated with 50µM CdCl2 and 18h-treated-roots and 24h-treated-shoots were harvested. Total RNA was then prepared and analyzed using the Affymetrix-Arabidopsis ATH1GeneChip array. Three biological replicates were performed for each sample. Different comparisons were performed as follows: 18h Cd-treated wild type roots versus untreated wild type roots; 24h Cd-treated wild type shoots versus untreated wild type shoots; 18h Cd-treated oas-a1.1 roots versus untreated oas-a1.1 roots; 24h Cd-treated oas-a1.1 shoots versus untreated oas-a1.1 shoots; 18h Cd-treated oas-a1.1 roots versus 18h Cd-treated wild type roots; 24h Cd-treated oas-a1.1 shoots versus 24h Cd-treated wild type shoots

			Action	DRS
	Fraction26.mgf	Mgf
	Fraction26.raw	Raw
	Peak5.mgf	Mgf
	Peak5.raw	Raw
	Results.mzid.gz	Mzid