Dataset Information

Revealing Melanoma Cell Proteome Transformations with Silver Nanoparticles: An Innovative Comparative Study

ABSTRACT: Addressing the challenges of melanoma, including its aggressive nature and treatment resistance. This study explores the application of silver nanoparticles (AgNPs) synthesized via conventional and green methods. By examining proteomic changes in A375 melanoma cells, we identify key disruptions in cell viability and gene activity. The DIA MS/SWATH MS analysis generates a set of differentially expressed proteins with respect to AgNPs. This allows us to identify new potential regulatory mechanisms and molecular pathways. AgNP exposure leads to oxidative stress, mitochondrial dysfunction, and activation of apoptosis pathways, revealing the potential of AgNPs in melanoma treatment. This comprehensive analysis highlights the promise of eco-friendly nanoparticle synthesis in developing effective anti-cancer therapies.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Skin

DISEASE(S): Melanoma

SUBMITTER: Jakub Faktor

LAB HEAD: Sachin Kote

PROVIDER: PXD058049 | Pride | 2025-05-07

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	230616_SPROT_HUMAN.fasta	Fasta
	Anotation_for_Skyline_file.csv	Csv
	DDA_ColloidalAgNPs_24_1.raw	Raw
	DDA_ColloidalAgNPs_24_2.raw	Raw
	DDA_ColloidalAgNPs_24_3.raw	Raw

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Publications

DIA/SWATH-Mass Spectrometry Revealing Melanoma Cell Proteome Transformations with Silver Nanoparticles: An Innovative Comparative Study.

Martano Simona S Faktor Jakub J Kote Sachin S Cascione Mariafrancesca M Di Corato Riccardo R Faktorova Dagmar D Semeraro Paola P Rizzello Loris L Leporatti Stefano S Rinaldi Rosaria R De Matteis Valeria V

International journal of molecular sciences 20250226 5

Melanoma is an aggressive cancer with rising incidence and high mortality rates, largely due to chemotherapy resistance and molecular dysregulation. Nanotechnology, particularly silver nanoparticles (AgNPs), has emerged as a promising therapeutic avenue because of the nanoparticles' ability to induce oxidative stress and apoptosis in cancer cells. However, conventional colloidal AgNPs lack selectivity, often causing significant damage to healthy cells. In this study, we introduce a green synthes ...[more]

PMID: 40076651

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