Dataset Information

A Class 1 OLD family nuclease encoded by Vibrio cholerae is countered by a vibriophage-encoded direct inhibitor

ABSTRACT: Bacteria are constantly threatened by their viral predators (phages), which has resulted in the development of defense systems for bacterial survival. One family of defense systems found widely across bacteria are OLD (for overcoming lysogeny defect) family nucleases. Despite recent discoveries regarding Class 2 and 4 OLD family nucleases and how phages overcome them, Class 1 OLD family nucleases warrant further study as there has only been one anti-phage Class 1 OLD family nuclease described to date. Here, we identify the Vibrio cholerae-encoded Class 1 OLD family nuclease Vc OLD and describe its disruption of genome replication of the lytic vibriophage ICP1. Furthermore, we examine its in vitro activity, identifying Vc OLD as a DNA nickase. Finally, we identify the first direct inhibitor of a Class 1 OLD family nuclease, the ICP1-encoded Oad1. Our research further illuminates Class 1 OLD family nucleases’ role in phage defense and explores the dynamic arms race between V. cholerae and its predatory phage ICP1.

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ORGANISM(S): Escherichia Coli

SUBMITTER: Kimberley Seed

LAB HEAD: Kimberley Seed

PROVIDER: PXD058617 | Pride | 2025-09-15

REPOSITORIES: Pride

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A DNA nicking Class 1 OLD family nuclease mediates phage defense in Vibrio cholerae and is countered by a phage-encoded inhibitor.

Patel Kishen M KM Seed Kimberley D KD

Nucleic acids research 20250801 16

Bacteria are constantly threatened by their viral predators (phages), which has resulted in the development of defense systems for bacterial survival. One family of defense systems found widely across bacteria are OLD (for overcoming lysogenization defect) family nucleases. Despite recent discoveries regarding Class 2 and 4 OLD family nucleases and how phages overcome them, Class 1 OLD family nucleases remain underexplored, with only one antiphage Class 1 OLD family nuclease described to date. H ...[more]

PMID: 40874590

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			Action	DRS
	KMP16_1of4.mgf	Mgf
	KMP16_1of4.raw	Raw
	KMP16_2of4.mgf	Mgf
	KMP16_2of4.raw	Raw
	KMP16_3of4.mgf	Mgf