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TMT proteomics of Cmtr1 cKO activated CD4 T cells

ABSTRACT: We aim to find the gene-specific effects of Cmtr1 KO in mouse CD4 T cells. TMT proteomics datasets were generated to find out which proteins are dependent on CMTR1 for their expression.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell, Lymph Node

SUBMITTER: Alison Galloway

LAB HEAD: Victoria H. Cowling

PROVIDER: PXD058851 | Pride | 2025-10-16

REPOSITORIES: Pride

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			Action	DRS
	2024.cmtr1.act_proteomics_limma.csv	Csv
	AG-Cmtr1-Frac1.raw	Raw
	AG-Cmtr1-Frac10.raw	Raw
	AG-Cmtr1-Frac11.raw	Raw
	AG-Cmtr1-Frac12.raw	Raw

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Publications

CMTR1 directs mitochondrial dynamics during T cell activation through epitranscriptomic regulation of splice isoforms.

Galloway Alison A Knop Katarzyna K Gomez-Moreira Carolina C Xavier Vanessa V Thomson Sarah S Yoshikawa Harunori H Suska Olga O Lukoszek Radoslaw R Kaskar Aneesa A Lamond Angus I AI MacVicar Thomas T Cowling Victoria H VH

Cell reports 20251013 10

During T cell activation, mitochondrial biogenesis and cellular metabolism are altered to meet the elevated energy demands of protein synthesis, rapid proliferation, and effector T cell function. The mechanisms coupling mitochondrial dynamics to T cell status are unclear. Here, we report that RNA cap methyltransferase 1 (CMTR1) is induced in activated T cells, methylating the first nucleotide on mRNA and U2 small nuclear RNA (snRNA), a component of the spliceosome. Using transcriptomic analyses, ...[more]

PMID: 41091598

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