The secretome of the white-rot basidiomycete Abortiporus biennis LGAM 436 can cause significant modifications to polystyrene structure

ABSTRACT: This study focused on investigating the biocatalytic potential of the white-rot basidiomycete Abortiporus biennis LGAM 436 to modify the structure of different types of PS, including amorphous film with atactic stereochemistry and commercial EPS foam. The selection of this specific strain was mainly based on a previous report by our group, focusing on the isolation of a laccase secreted by A. biennis LGAM 436, which is capable of reducing the number-average molecular weight of PS by 20% (10.1016/j.chemosphere.2022.137338). To expedite the process of whole cell biocatalysis, we also evaluated the addition of the phenol-rich olive-oil mill wastewater effluent (OOMW); a byproduct which can act as an inducer of oxidative enzymes such as laccases and LiPs (10.1016/j.jenvman.2016.02.042). Following the assessment of PS degradation through analyses of molar mass, the emergence of new functional groups, and alterations in surface morphology, we sought to elucidate the enzymatic activities expressed in the presence of PS. This was achieved after the secretome assessment of the basidiomycete via proteomics studies, which will pave the way for the heterologous expression of novel enzymatic activities that can synergistically act to modify and degrade polyolefin structures.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Abortiporus Biennis

SUBMITTER: Romanos Siaperas  

LAB HEAD: Evangelos Topakas

PROVIDER: PXD058977 | Pride | 2025-04-24

REPOSITORIES: Pride