Dataset Information

Liver tumor tissues extracted from a mouse model of hepatocellular carcinoma were subjected to proteomics

ABSTRACT: The highly metastatic MHCC-97H HCC cell line was orthotopic transplanted into the liver of nude mice to induce orthotopic tumor formation, and lung metastasis was observed. This experimental model not only reproduces the metastasis process of HCC in vivo, but also provides an important biological material for studying the tumor microenvironment and metastasis mechanism. Subsequently, we collected liver cancer tissues, and further conducted in-depth proteomic analysis of the collected liver tumor tissue samples, in order to reveal the key protein expression changes and potential signaling pathway regulation mechanisms during tumor development and metastasis, and lay the foundation for subsequent targeted therapy research.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: Hui Zhang

LAB HEAD: Hui zhang

PROVIDER: PXD059613 | Pride | 2026-06-08

REPOSITORIES: Pride

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Publications

Dysregulated Proline Metabolism Exacerbates Hepatocellular Carcinoma Metastasis via EPRS1-Mediated mRNA Translation.

Zhang Hui H Gu Leirong L Su Xiameng X Chen Wanjin W Tan Ming M Yu Haibo H Zhou Hongzhong H Gao Tingting T Wang Zhiling Z Chen Xinyan X Chen Weixian W Chen Juan J Cheng Shengtao S

Cancer communications (London, England) 20260514

<b>Background:</b> Metabolic reprogramming is a hallmark of hepatocellular carcinoma (HCC), and amino acid reprogramming plays an important role in its metastatic progression. However, the function of amino acid reprogramming in HCC metastatic progression remains unclear. This study aimed to elucidate the function and mechanism of amino acid reprogramming, particularly focusing on proline metabolism, in driving HCC metastasis. <b>Methods:</b> HCC cohort and multiple HCC models were enrolled to i ...[more]

PMID: 42147354

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Project description:Hepatectomy generally offers the best chance of long-term survival for patients with hepatocellular carcinoma (HCC). Many studies have shown that hepatectomy accelerates tumor metastasis, but the mechanism remains unclear. In this study, palliative hepatectomy was performed in an orthotopic nude mice model of HCC (MHCC97H) with high metastatic potential. Using Human Tumor Metastasis Microarray, we screened the metastasis-related genes in tumor tissues following palliative resection, and found that Metastasis suppressor 1 (MTSS1) located in the central position of gene function net of residual HCC; MTSS1 was up-regulated in residual tumor after palliative resection. Further studies found that MTSS1 enhanced the metastasis of residual HCC. In hepatitis B-related HCC patients undergone palliative hepatectomy, those with higher MTSS1 mRNA expression accompanied by the activation of matrix metalloproteinase 2 (MMP2) in residual HCC, had earlier residual HCC detection after hepatectomy and poorer survival when compared to those with lower MTSS1 level. In different cell lines, the levels of MTSS1 mRNA increased in parallel with metastatic potential. MTSS1 down regulation via siRNA decreased MMP2 activity, reduced invasive potentials of HCC by 28.9% in vitro, and averted the deteriorated lung metastatic extent in vivo. In conclusion, the poor prognosis of hepatitis B-related HCC patients following palliative hepatectomy associates with elevated MTSS1 mRNA expression; therefore, MTSS1 may provide a new research field for HCC diagnosis and treatment. Eighteen nude mice bearing HCC xenografts were randomized into three groups 14 days after orthotopic implantation: palliative resection group (mice received partial HCC resection with preservation of 2 mm tumor pedicles), sham operation group (mice only undergone an exposure of liver but without resection), and blank control group (mice without further surgical intervention). All mice were sacrificed by cervical dislocation 14 days following palliative resection based on pre-experimental results. The first time surgically removed HCC tissues were named tumor tissues T1; the second time surgically removed HCC tissues from sham operation group were named tumor tissues T2; Tissues from blank control group were named tumor tissues T3; Tissues from palliative resection group were named tumor tissues T4 that was residual HCC. Randomly selected 5 tumor specimens from each group were used for the screen of genes related to metastasis by microarray techniques.

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