Inferring the composition of the blood plasma proteome by a human proteome distribution atlas
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ABSTRACT: The plasma proteome is maintained by the influx and efflux of proteins from surrounding organs and cells. To quantify the impact surrounding organs and cells have on the plasma proteome, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in human plasma. To quantify the extent different organs and cells contribute to the plasma proteome composition, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in human plasma in healthy and diseased conditions. We constructed an extensive human proteome atlas from 18 vascularized organs and the 8 most abundant cell types in blood. The atlas was interfaced with previous RNA and protein atlases to objectively define proteome wide protein-organ associations to enable both the inference of origin and the reproducible quantification of organ-specific proteins in plasma. We demonstrate that the resource can determine disease-specific quantitative changes of organ-enriched protein panels in six separate patient cohorts including sepsis, pancreatitis, and myocardial injury. The strategy can be extended to other diseases to advance our understanding of the processes contributing to plasma proteome dynamics.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Heart, Spleen, Brain, B Cell, Blood Plasma, Monocyte, Leukocyte, Liver, Lung, Kidney, Erythrocyte, Blood Platelet, T Cell, Skin, Neutrophil, Colon, Bone Marrow, Macrophage
DISEASE(S): Viral Infectious Disease,Bacterial Infectious Disease,Pancreatitis,Heart Disease
SUBMITTER:
Christofer Karlsson
LAB HEAD: Prof. Johan Malmström
PROVIDER: PXD059640 | Pride | 2025-04-08
REPOSITORIES: Pride
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