Proteomics

Dataset Information

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Inferring the composition of the blood plasma proteome by a human proteome distribution atlas


ABSTRACT: The plasma proteome is maintained by the influx and efflux of proteins from surrounding organs and cells. To quantify the impact surrounding organs and cells have on the plasma proteome, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in human plasma. To quantify the extent different organs and cells contribute to the plasma proteome composition, we developed a mass spectrometry-based proteomics strategy to infer the origin of proteins detected in human plasma in healthy and diseased conditions. We constructed an extensive human proteome atlas from 18 vascularized organs and the 8 most abundant cell types in blood. The atlas was interfaced with previous RNA and protein atlases to objectively define proteome wide protein-organ associations to enable both the inference of origin and the reproducible quantification of organ-specific proteins in plasma. We demonstrate that the resource can determine disease-specific quantitative changes of organ-enriched protein panels in six separate patient cohorts including sepsis, pancreatitis, and myocardial injury. The strategy can be extended to other diseases to advance our understanding of the processes contributing to plasma proteome dynamics.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart, Spleen, Brain, B Cell, Blood Plasma, Monocyte, Leukocyte, Liver, Lung, Kidney, Erythrocyte, Blood Platelet, T Cell, Skin, Neutrophil, Colon, Bone Marrow, Macrophage

DISEASE(S): Viral Infectious Disease,Bacterial Infectious Disease,Pancreatitis,Heart Disease

SUBMITTER: Christofer Karlsson  

LAB HEAD: Prof. Johan Malmström

PROVIDER: PXD059640 | Pride | 2025-04-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CK_P1906_020.raw Raw
CK_P1906_021.raw Raw
CK_P1906_022.raw Raw
CK_P1906_023.raw Raw
CK_P1906_024.raw Raw
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