Transcription profiling of wild type and hog1 Candida albicans subjected to environmental stresses that are known to activate the Hog1 Stress-activated Protein Kinase

ABSTRACT: Based on studies in S. pombe (Chen et al., 2003), we predicted that conditions which activate C. albicans Hog1 would result in the induction of a common set of genes that are regulated by this SAPK (Smith et al., 2004). Hence in this study we compared the global transcriptional responses of wild-type and hog1 C. albicans cells to environmental stresses that are known to activate the Hog1 SAPK. We compared the homozygous hog1/hog1 null mutant (JC50) with the isogenic HOG1 reintegrant (hog1/hog1/HOG1: JC52) because this controlled for any secondary mutations that might have been introduced during the construction of the null mutant. We have shown that this reintegrant is indistinguishable from its parental wild-type strain RM1000 (HOG1/HOG1) with respect to their stress phenotypes (Smith et

INSTRUMENT(S): ScanArray Lite [PerkinElmer]

ORGANISM(S): Candida albicans

SUBMITTER: Brice Enjalbert 

PROVIDER: E-MEXP-1134 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress