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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Jacobsen2010

ABSTRACT: A pre-culture in NGY (overnight 30M-0C) was used to inoculate RPMI-1640 medium to an initial optical density at 600 nm (OD600) of 0.05. Cells were then grown to mid exponential phase (OD600 = 0.5M-^V0.6) at the appropriate temperature (25M-0C or 35M-0C) before the culture was

split into two aliquots and fluconazole (32 ?g mlM-^V1 final concentration) added to one; the other serving as the control. After 2 h the cells were harvested by centrifugation and flash

frozen in liquid nitrogen. Three independent cultures were grown for RNA extraction for each isolate at each condition. RNA was prepared from cell pellets with TRIZOLM-. reagent (Invitrogen) and a mechanical disruption method.

ORGANISM(S): Candida albicans

SUBMITTER: AFG AFG

PROVIDER: E-MEXP-3000 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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