Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

16 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Candida albicans wild type strain BWP17 and mutant hac1 treated with DTT or tunicamycin to investigate the effect of the Unfolded Protein Response of genome-wide expression patterns

ABSTRACT: Candida albicans wild type strain BWP17 and mutant hac1 were submitted either to DTT or tunicamycin, both agents being able to trigger the Unfolded Protein Response.

ORGANISM(S): Candida albicans

SUBMITTER: AFG AFG

PROVIDER: E-MEXP-1307 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of wild type and hog1 Candida albicans subjected to environmental stresses that are known to activate the Hog1 Stress-activated Protein Kinase

Project description:Based on studies in S. pombe (Chen et al., 2003), we predicted that conditions which activate C. albicans Hog1 would result in the induction of a common set of genes that are regulated by this SAPK (Smith et al., 2004). Hence in this study we compared the global transcriptional responses of wild-type and hog1 C. albicans cells to environmental stresses that are known to activate the Hog1 SAPK. We compared the homozygous hog1/hog1 null mutant (JC50) with the isogenic HOG1 reintegrant (hog1/hog1/HOG1: JC52) because this controlled for any secondary mutations that might have been introduced during the construction of the null mutant. We have shown that this reintegrant is indistinguishable from its parental wild-type strain RM1000 (HOG1/HOG1) with respect to their stress phenotypes (Smith et al., 2004) and their expression of stress genes (Supplementary Data). Three conditions were chosen for transcript profiling: osmotic stress imposed by 0.3 M NaCl, oxidative stress imposed by 5 mM H2O2, and heavy metal stress imposed by 0.5 mM CdSO4. Each of these treatments stimulates the phosphorylation and nuclear accumulation of this Hog1 SAPK within a 10-min time frame (Smith et al., 2004). Furthermore, significant differences in stress regulated gene expression are observed within this time scale (Enjalbert et al., 2003; Smith et al., 2004). Hence, we analyzed the C. albicans transcriptome after a 10-min exposure to each stress condition. Although some stress genes might be missed by analyzing a single time point, most C. albicans stress genes are induced within 10 min (Enjalbert et al., 2003). At least four independent biological replicates were analyzed for each condition

2007-07-05 | E-MEXP-1134 | biostudies-arrayexpress

Transcription profiling of Candida albicans wildtype and hsf1 mutant cells after heatstress

Project description:effect of heatstress on wildtype and hsf1 mutant cells

2009-12-17 | E-MEXP-2044 | biostudies-arrayexpress

Transcription profiling of Candida albicans in response to a sudden availability of glucose in the extracellular medium, having previously been grown on a non-fermentable carbon source

Project description:We investigated how Candida albicans adapts its transcriptional pattern to a sudden availability of glucose in the extracellular medium, having previously grown on a non-fermentable carbon source.

2008-06-01 | E-MEXP-1151 | biostudies-arrayexpress

Transcription profiling wild type C. albicans after treatment with stress-inducing doses of acetic acid

Project description:Transcript profiling was performed using a wild-type C. albicans strain (CaI8+CIp10). Cells were cultured in 50 ml SC-pH3.0 to a cell density of 1x10^7 cells per ml and then either treated with control (0 mM acetic acid) or stress inducing (20 mM acetic acid) doses. Cells from the same culture were harvested after 300 min treatment. Three independent biological replicates were obtained for each condition.

2008-07-31 | E-MEXP-1633 | biostudies-arrayexpress

Transcription profiling by array of Candida albicans to compare Gcn4 and Gcn2 regulons

Project description:comparison of the Gcn4 and Gcn2 regulons by transcript profiling

2010-05-13 | E-MEXP-1212 | biostudies-arrayexpress

Transcription profiling by array of Candida albicans tup1, nrg1 and ssn6 mutants

Project description:Profilings of the Candida albicans mutant: tup1, nrg1, ssn6 (smooth and wrinkled phenotypes)

2010-05-14 | E-MEXP-1217 | biostudies-arrayexpress

Transcription profiling of Candida albicans clinical isolates grown under identical growth conditions in NGY medium

Project description:Comparison of clinical isolates grown under identical growth conditions

2009-02-11 | E-MEXP-1931 | biostudies-arrayexpress

Transcription profiling of Candida albicans clinical isolates

Project description:Comparison of 20 clinical isolates of Candida albicans grown under identical growth conditions (biofilm growth conditions in RPMI 1640/10% serum)

2009-02-11 | E-MEXP-1933 | biostudies-arrayexpress

Transcription profiling time series of Candida albicans treated with different doses of acetic acid

Project description:Wild type Candida albicans (CAI8 containing CIp10) cultures were grown in triplicate at 1 x 107 cells ml-1 in 50 ml SC-pH3.0 broth. Cultures were then treated with 0, 20, 120 or 300<br>mM acetic acid. Cells were harvested after zero, 10, 30, 60, 120, 210 and 300 min treatment. RNA isolated from triplicate biological replicates were compared with a standard pooled control (untreated cells).

2008-07-31 | E-MEXP-1645 | biostudies-arrayexpress

Transcription profiling of Candida albicans wild type and mnl1 cells after treatment with acetic acid

Project description:Wt vs mnl1 cells after 300 min treatment with 20 mM acetic acid in SC-pH3.0

2008-07-31 | E-MEXP-1641 | biostudies-arrayexpress