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S-nitrosoproteome analysis of rice leaves in response to Magnaporthe oryzae infection

ABSTRACT: S-nitrosoproteome of leaves obtained from two rice cultivars Dongjin (relatively resistant) and Nipponbare (susceptible) to Magnaporthe oryzae strain PO6-6 was investigated using a iodo-TMT six plex kit. MS/MS data was acquired using two MS intruments: QExactive™ Orbitrap High-Resolution Mass Spectrometer (MS) (Thermo Fisher Scientific, MA, USA) or with Orbitrap Exploris™ 240 The labeling was performed in the following order: iodoTMT reagent 126: Dongjin mock iodoTMT reagent 127: Dongjin infected iodoTMT reagent 128: Dongjin mock+infected+GSNO iodoTMT reagent 129: Nipponbare mock iodoTMT reagent 130: Nipponbare infected iodoTMT reagent 131: Nipponbare mock+infected+GSNO

INSTRUMENT(S):

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Leaf

SUBMITTER: Ravi Gupta

LAB HEAD: Ravi Gupta

PROVIDER: PXD059677 | Pride | 2025-12-20

REPOSITORIES: Pride

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			Action	DRS
	TMT_RG_SN_01_OE240.raw	Raw
	TMT_RG_SN_01_QE.raw	Raw
	TMT_RG_SN_02_OE240.raw	Raw
	TMT_RG_SN_02_QE.raw	Raw
	TMT_RG_SN_03_OE240.raw	Raw

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Publications

S-Nitrosoproteome Analysis of Rice Leaves Highlights the Possible Roles of Superoxide Dismutase in Resistance Against Magnaporthe Oryzae.

Munir Adeela A Min Cheol Woo CW Wang Yiming Y Kim Sun Tae ST Gupta Ravi R

Physiologia plantarum 20250901 5

Nitric oxide (NO) is a key signaling molecule and is known to modulate diverse physiological processes, including defense responses against pathogens. However, the molecular mechanism underlying NO-induced plant immunity remains largely elusive. Here, we investigated the dynamics of NO biosynthesis and its downstream signaling through S-nitrosoproteome analysis of two rice cultivars, Dongjin (DJ, resistant) and Nipponbare (NIP, susceptible), in response to Magnaporthe oryzae, the causative agent ...[more]

PMID: 40976710

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