Dataset Information

Proteome analysis of T24 bladder cancer cell nuclear extracts after tailored chemical interventions

ABSTRACT: Bladder cells are constantly exposed to multiple chemical stressors stemming from urine, including xenobiotics and bioactive metabolites. Even if not completely understood at the molecular level, a significant adaptability to these challenges is a prerequisite for bladder cells and enables the maintenance of physiological function. Here untargeted profiling was applied to grasp changes in bladder cells proteome upon application of tailored chemical interventions. To focus on regulatory events observable in the nuclear and peri-nuclear compartments, nuclear extracts were generated after incubation with different molecules enabling regulation of oxidative stress (1 µM MitoTEMPOL, 500 µM H2O2), autophagy (10 nM Bafilomycin A1), cytoskeletal integrity (100 µM CK666), and metabolism (1 mM 2-desoxyglucose). T24 cells were incubated with the respective treatments for 24 hours, after which nuclear proteins were isolated, digested and analyzed using a timsTOF Pro mass spectrometer (Bruker). Identification as well as label-free quantification (LFQ) of proteins and statistical analyses were performed using publicly available software package MaxQuant 1.6.17.0.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urinary Bladder Carcinoma Cell Line, T-24 Cell

SUBMITTER: Christopher Gerner

LAB HEAD: Christopher Gerner

PROVIDER: PXD060036 | Pride | 2025-08-04

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	22-Prot-0283_RD7_1_8517.d.rar	Other
	22-Prot-0283_RD7_1_8570.d.rar	Other
	22-Prot-0284_RD8_1_8518.d.rar	Other
	22-Prot-0284_RD8_1_8571.d.rar	Other
	22-Prot-0285_RE1_1_8555.d.rar	Other

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Publications

Retrograde rearrangement of mitochondria correlates with nuclear deformation and genotoxic damage.

Jobst Maximilian M Crudo Francesco F Marko Doris D Bileck Andrea A Meier-Menches Samuel Matthias SM Gerner Christopher C Del Favero Giorgia G

iScience 20250619 8

The elucidation of molecular mechanisms underlying DNA damage and repair belongs to the fundamental questions of pathophysiology and toxicology. Increasing attention is focusing on the role of mechanical stress exerted on the nucleus and its function as a mechanosensor. Hypothesizing that physical cues arising from the intracellular rearrangements could contribute to the genotoxic damage, we observed that the retrograde relocalization of the mitochondria coincides with increased nuclear stiffnes ...[more]

PMID: 40727930

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