Dataset Information

Latent infection of an active giant endogenous virus in a unicellular green alga

ABSTRACT: Latency is a common strategy in a wide range of viral lineages, but its prevalence in giant viruses remains unknown. Here we describe the activity and viral production from a 617 kbp integrated giant viral element in the model green alga Chlamydomonas reinhardtii. We resolve the integrated viral region using long-read sequencing and show that viral particles are produced and released in otherwise healthy cultures. A diverse array of viral-encoded selfish genetic elements are expressed during GEVE reactivation and produce proteins that are packaged in virions. In addition, we show that field isolates of Chlamydomonas sp. harbor latent giant viruses related to the C. reinhardtii GEVE that exhibit similar infection dynamics, demonstrating that giant virus latency is prevalent in natural host communities. Our work reports the largest temperate virus documented to date and the first active GEVE identified in a unicellular eukaryote, substantially expanding the known limits of viral latency.

INSTRUMENT(S):

ORGANISM(S): Nucleocytoviricota

SUBMITTER: Keith Ray

LAB HEAD: Dr. Frank O. Aylward

PROVIDER: PXD060118 | Pride | 2025-06-16

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	240516_Rep1_01_YA1_1_1128.d.zip	Other
	240516_Rep1_01_YA1_1_1128.mgf	Mgf
	240516_Rep1_01_YA1_1_1128.mzid	Mzid
	240516_Rep1_02_YA1_1_1129.d.zip	Other
	240516_Rep1_02_YA1_1_1129.mgf	Mgf

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Publications

Cryptic infection of a giant virus in a unicellular green alga.

Erazo-Garcia Maria P MP Sheyn Uri U Barth Zachary K ZK Craig Rory J RJ Wessman Petronella P Jivaji Abdeali M AM Ray W Keith WK Svensson-Coelho Maria M Cornwallis Charlie K CK Rengefors Karin K Brussaard Corina P D CPD Moniruzzaman Mohammad M Aylward Frank O FO

Science (New York, N.Y.) 20250515 6748

Latency is a common strategy in a wide range of viral lineages, but its prevalence in giant viruses remains unknown. In this work, we describe a 617-kilo-base pairs integrated giant viral element in the model green alga <i>Chlamydomonas reinhardtii</i>. We resolved the integrated viral genome using long-read sequencing, identified a putative polintovirus-like integrase, and show that viral particles accumulate primarily during the stationary growth phase. A diverse array of viral-encoded selfish ...[more]

PMID: 40208960

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