Dataset Information

Gut microbiota induce neutrophil activation that aggravates stroke pathology

ABSTRACT: Neutrophils, key players in the innate immune system, become activated following ischemic stroke. However, the influence of gut microbiota on neutrophil activation and its impact on inflammatory brain injury is not yet fully understood. In this study, we demonstrate that microbiota colonization in germ-free mice activates neutrophils and worsens disease outcomes. To investigate the phenotypic and molecular alterations in neutrophils, as well as their activity in stroke mice with either depleted or intact microbiota, we conducted a comparative proteomic analysis using liquid chromatography-based mass spectrometry. Our findings revealed that microbiota depletion leads to a downregulation of inflammatory proteins in circulating neutrophils and a reduction in neutrophil extracellular traps (NETs). A similar pattern was observed in neutrophils infiltrating the brain, accompanied by a decrease in infarct size and alleviated behavioral deficits.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Neutrophil, Blood

DISEASE(S): Brain Ischemia

SUBMITTER: Susmita Ghosh

LAB HEAD: Albert Sickmann

PROVIDER: PXD060229 | Pride | 2025-06-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Sample_Table.xlsx	Xlsx
	Stroke_blood_neutrophils.sne	Other
	Stroke_brain_neutrophils.sne	Other
	Stroke_mice_Blood.zip	Other
	Stroke_mice_brain.zip	Other

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Publications

Gut microbiota deficiency reduces neutrophil activation and is protective after ischemic stroke.

Tuz Ali A AA Ghosh Susmita S Karsch Laura L Antler Medina M Lakovic Vivian V Lohmann Sabrina S Lehmann Amber Hope AH Beer Alexander A Nagel Dennis D Jung Marcel M Hörenbaum Nils N Kaygusuz Viola V Qefalia Altea A Alshaar Belal B Amookazemi Niloufar N Bolsega Silvia S Basic Marijana M Siveke Jens T JT Heiles Sven S Grüneboom Anika A Lueong Smiths S Herz Josephine J Sickmann Albert A Hagemann Nina N Hasenberg Anja A Hermann Dirk M DM Gunzer Matthias M Singh Vikramjeet V

Journal of neuroinflammation 20250523 1

Neutrophils are readily activated immune cells after ischemic stroke in mice and patients. Still, the impact of gut microbiota on neutrophil activation and its influence on inflammatory brain injury remain undefined. We report that natural microbiota colonization of germ-free (GF) mice induces substantial neutrophil activation and deteriorates stroke pathology. The colonized Ex-GF stroke mice had considerably larger infarct sizes and higher sensorimotor deficits than GF littermates. Furthermore, ...[more]

PMID: 40410847

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