Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sex-specific extracellular protein composition derived from oligodendrocyte progenitor cells (OPC) under normoxic and hyperoxic conditions

ABSTRACT: Secretory proteins and extracellular vesicles (EVs) are increasingly recognized as important mediators of intercellular communication and stress response in the brain. Our analysis of the secretome from cell culture supernatants obtained after treating male and female derived primary mouse OPCs with hyperoxia (80% O2) for a 24 hours’ period, showed prominent sex-specific protein signatures.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Elke Hammer

LAB HEAD: Elke Hammer

PROVIDER: PXD060307 | Pride | 2025-09-29

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	190521_HF_EH_Neona_Ctrl_H_f_BR3.raw	Raw
	190521_HF_EH_Neona_Ctrl_H_m_BR3.raw	Raw
	190521_HF_EH_Neona_Ctrl_N_f_BR3.raw	Raw
	190521_HF_EH_Neona_Ctrl_N_m_BR3.raw	Raw
	190521_HF_EH_Neona_IP_H_f_BR1.raw	Raw

Items per page:

1 - 5 of 14

Publications

Sex-Specific Differences in the Secretome of Oligodendrocyte Progenitor Cells Post Hyperoxic Stress.

Sunny Donna Elizabeth DE Hammer Elke E Michalik Stephan S Völker Uwe U Heckmann Matthias M

Journal of extracellular biology 20250923 9

Cerebral oxygenation differences in the neonatal period of human preterm infants, along with sex-specific differences in combating oxidative stress, can lead to disruption of normal oligodendrocyte maturation and function, which in turn can differentially affect neuronal development and activity in the male and female brains. Secretory proteins and extracellular vesicles (EVs) are increasingly recognized as important mediators of intercellular communication and stress response in the brain. Our ...[more]

PMID: 40994720

Similar Datasets

Differential expressions of microRNAs under normoxic and hypoxic conditions

Project description:Outcome prediction classifiers were successfully constructed through expression profiling of a total of 1,329 miRNAs in MKN1, gastric cancer cell line under normoxic and hypoxic conditions. In the study presented here, MKN1 under normoxic and hypoxic conditions was used to acquire expression profiles of a total 1,329 unique miRNAs.

2014-04-18 | E-GEOD-56870 | biostudies-arrayexpress

Gene expression profile of normoxic versus hypoxic HeLa cells

Project description:To identify new primary HIF target genes we cultured HeLa cells under short period (6 hours) of mild hypoxia (1% oxygen) Hypoxia-induced gene expression in HeLa cells was measured after 6h exposure to 1% oxygen and compare to normoxic

2011-11-08 | E-GEOD-33521 | biostudies-arrayexpress

Changes in the oligodendrocyte cell progenitor cell proteome with ageing

Project description:Following CNS demyelination, adult oligodendrocyte progenitor cells (OPCs) can differentiate into new myelin-forming oligodendrocytes in a regenerative process called remyelination. While remyelination is very efficient in young adults, its efficiency declines progressively with ageing. Here we performed proteomic analysis on OPCs isolated acutely from the brains of neonate, young and aged female rats. Approximately 60% of the proteins are expressed at different levels in OPCs from neonates compared to their adult counterparts. The amount of myelin-associated and cell adhesion proteins are increased with age, while cholesterol-biosynthesis, transcription factors and cell cycle proteins decreased. Our experiments provide the first ageing OPC rodent proteome, revealing the distinct features of neonatal and adult OPCs, and providing new insights into why remyelination efficiency declines with ageing and potential roles for aged OPCs in other neurodegenerative diseases.

2022-02-25 | PXD010383 | Pride

Changes in the oligodendrocyte cell progenitor cell proteome with ageing

2020-05-22 | PXD013708 | Pride

Hypoxia modulates EWS-FLI1 transcriptional signature and enhances malignant properties of Ewing’s tumor cells in vitro

Project description:Hypoxia is an important condition in the tumor cell microenvironment and approximately 1-1.5% of the genome is transcriptionally responsive to hypoxia with hypoxia-inducible factor-1 (HIF-1) as a major mediator of transcriptional activation. Tumor hypoxia is associated with a more aggressive phenotype of many cancers in adults, but data on pediatric tumors are scarce. By immunohistochemical analysis, HIF-1α expression was readily detectable in 18/28 primary Ewing´s sarcoma family tumors (ESFT), a group of highly malignant bone-associated tumors in children and young adults, which encouraged us to study the effect of hypoxia on ESFT cell lines in vitro. Many tumors are profoundly hypoxic and multiple studies have demonstrated that hypoxic tumors have a poorer prognosis than non-hypoxic tumors. The cellular adaptations of cancer cells to hypoxia have been shown to profoundly influence transcriptional regulation. Intriguingly, we found that EWS-FLI1 protein expression, which characterizes ESFT, is up-regulated by hypoxia in a HIF-1α-dependent manner. Hypoxia modulated the EWS-FLI1 transcriptional signature relative to normoxic conditions. Both synergistic as well as antagonistic transcriptional effects of EWS-FLI1 and of hypoxia were observed. Consistent with alterations in the expression of metastasis related genes, hypoxia stimulated the invasiveness and soft-agar colony formation of ESFT cells in vitro. Our data represents the first transcriptome analysis of hypoxic ESFT cells and identifies hypoxia as an important microenvironmental factor modulating EWS-FLI1 expression and target gene activity with far-reaching consequences for the malignant properties of ESFT. Experiment Overall Design: We analysed the consequences of hypoxia on gene expression in two ESFT cell lines (SK-N-MC, TC252) grown as multicellular spheroids and as adherent monolayers.

2010-02-05 | E-GEOD-19197 | biostudies-arrayexpress

Gene expression profile of HIF-1Î± silenced or non-silenced human DCs stimulated with A. fumigatus under normoxic or hypoxic conditions

Project description:DCs play a central role for the immune response against the mold Aspergillus fumigatus. Hypoxic microenvironments occur during infection with A. fumigatus. Hypoxia and signaling via hypoxia inducible factor 1Î± may modulate the response of DCs; however, the role in fungal infections is unclear. We used microarrays to determine the influence of hypoxia and HIF-1Î± signaling on the immune response of human DCs towards A. fumigatus. HIF-1Î± silenced or non-silenced, human monocyte-derived DCs were cultivated for 6 h in normoxia or hypoxia (1 % O2) without stimulation or stimulated with inactivated germ tubes of A. fumigatus. Three independent experiments with DCs derived from different blood donors were performed. RNA was extracted and hybridized on Affymetrix microarrays.

2015-11-01 | E-GEOD-60729 | biostudies-arrayexpress

The role of CD44 in the naked-mole rat oligodendrocyte progenitor cells

Project description:The role of CD44 in naked-mole rats (NMR) oligodendrocyte progenitor cells (OPCs) were evaluated by comparing the transcriptome of control and CD44-knockdown NMR OPCs.

2022-03-31 | E-MTAB-9166 | biostudies-arrayexpress

Rapid and robust generation of functional oligodendrocyte progenitor cells from epiblast stem cells

Project description:Stem cell biology has garnered much attention due to its potential to impact human health through disease modeling and cell replacement therapy. This is especially pertinent to myelin-related disorders such as multiple sclerosis and leukodystrophies where restoration of normal oligodendrocyte function could provide an effective treatment. Progress in myelin repair has been constrained by the difficulty in generating pure populations of oligodendrocyte progenitor cells (OPCs) in sufficient quantities. Pluripotent stem cells theoretically provide an unlimited source of OPCs but significant advances are currently hindered by heterogeneous differentiation strategies that lack reproducibility. Here we provide a platform for the directed differentiation of pluripotent mouse epiblast stem cells (EpiSCs) through a defined series of developmental transitions into a pure population of highly expandable OPCs in ten days. These OPCs robustly differentiate into myelinating oligodendrocytes both in vitro and in vivo. Our results demonstrate that pluripotent stem cells can provide a pure population of clinically-relevant, myelinogenic oligodendrocytes and offer a tractable platform for defining the molecular regulation of oligodendrocyte development, drug screening, and potential cell-based remyelinating therapies. 6 total samples were analyzed. Pluripotent epiblast stem cells (EpiSCs) were differentiated to patterned neural rosettes, oligodendrocyte progenitor cells (OPCs), and oligodendrocytes. OPCs and oligodedrocytes were analyzed at two separate passages (3 and 11).

2011-10-02 | E-GEOD-31562 | biostudies-arrayexpress

Oligodendrocyte progenitor cells become regionally diverse and heterogeneous with age

Project description:Oligodendrocyte progenitor cells (OPCs), which differentiate into myelinating oligodendrocytes during central nervous system (CNS) development, are the main proliferative cells in the adult brain. OPCs are conventionally considered a homogeneous population, particularly with respect to their electrophysiological properties, but this has been debated. We show, by using single-cell electrophysiological recordings, that OPCs start out as a homogeneous population, but become functionally heterogeneous, varying both within and between brain regions and with age. These electrophysiological changes in OPCs correlate with the differentiation potential of OPCs; thus, they may underlie the differentiational differences in OPCs between regions and likewise differentiation failure with age.

2019-01-15 | GSE121083 | GEO

Oligodendrocyte precursor cells facilitate neuronal lysosome release

Project description:Oligodendrocyte precursor cells (OPCs) shape brain function through complex mechanisms. We found that OPCs facilitate neuronal lysosome exocytosis by contacting neuronal somata. Reduced number or process branching of OPCs decreased these contacts, accompanied with lysosome accumulation and altered metabolism in neurons, and more senescent neurons with age. In Alzheimer's mouse models, OPC-neuron contacts were fewer, and lysosomes accumulated. Our findings are relevant for preventing aging-related pathologies and treatment of neurodegenerative diseases.

2025-01-14 | GSE285995 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data