Dataset Information

Aloin Protects Against UVB-Induced Apoptosis

ABSTRACT: This study comprehensively investigates the photoprotective effects of aloin against UVB-induced damage in HaCaT cells, elucidating its antioxidant capacity and its role in preventing cellular apoptosis. The proteomic effect of 50 μg/mL aloin on HaCaT cell damage induced by UVB was evaluated through comparative protein expression. Compared to control cells, UVB irradiation significantly altered protein expression in HaCaT cells, with 64 proteins upregulated and 236 proteins downregulated. In cells treated with both UVB and aloin, 79 proteins were upregulated and 84 proteins were downregulated compared to the UVB-only group. Proteomic analysis showed that aloin modulated the expression of proteins involved in critical signaling pathways, including PI3K-Akt, p53, TGF-β and pathways in cancer, promoting cell survival. Aloin upregulated proteins associated with cell cycle regula-tion and antioxidant responses, such as CCND3, GSTM4, GNA12, SKIL, YWHAZ, and PKN3 while downregulating pro-apoptotic protein FOXO3.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Keratinocyte

SUBMITTER: Yu-Pei Chen

LAB HEAD: Yu-Pei Chen

PROVIDER: PXD060808 | Pride | 2025-08-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20240819_154727_sp_MJ20240718065_PP20240726542.sne	Other
	Hg22CYP_BK_D_1_16.raw	Raw
	Hg22CYP_BK_D_2_17.raw	Raw
	Hg22CYP_BK_D_3_18.raw	Raw
	Hg22CYP_UV_AA_1_22.raw	Raw

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Publications

Aloin protects against UVB-induced apoptosis by modulating integrated signaling pathways.

He Qi Q Chen Yu-Pei YP Wu Chun C Wu Hongtan H Li Fei F Li Mingyu M Chen Fangfang F

Frontiers in pharmacology 20250711

Aloin, an anthraquinone compound, is naturally abundant in the <i>Aloe</i>. This study comprehensively investigates the photoprotective effects of aloin against UVB-induced damage in HaCaT cells, elucidating its antioxidant capacity and its role in preventing cellular apoptosis. Aloin demonstrated significant antioxidant activity in ABTS and DPPH assays, with a dose-dependent reduction in intracellular reactive oxygen species levels as evidenced by fluorescence analysis. Western blot analysis re ...[more]

PMID: 40717965

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