Dataset Information

Protein Metabolism Underlying Heat Tolerance in Contrasting Creeping Bentgass Lines: Insights From Gel-Free Proteomics

ABSTRACT: One of the major disfunctions that occurs in heat-stressed plants is enhanced protein damage and a consequent decline in cellular protein content. Creeping bentgrass (Agrositis stolonifera L.) is an economically important perennial grass species which is largely used on high value turf areas but experiences frequent damage due to heat stress. Several promising experimental lines of creeping bentgrass showed various tolerance levels to heat stress, with differential responses observed in physiological traits, total protein content and rates of protein degradation. The ubiquitin-proteasome system (UPS) plays a crucial role in the removal of damaged proteins, and there is a critical need to better understand the changes in protein accumulations and degradation via the UPS that occur during heat stress. Hence, we aimed to estimate change in global protein accumulations by performing gel-free proteomics as well as identify proteins that have been polyubiquitinated and targeted to the UPS pathway via polyubiquitin-omics in contrasting creeping bentgrass lines exposed to heat stress. It was found that heat-tolerant S11 729-10 was able to maintain less severe downregulation of proteins involved in the light reactions of photosynthesis, while enhancing the upregulation of antioxidant proteins, particularly during the later phase of stress. These contributed to its improved physiological performance including greater cell membrane integrity as well as healthier light harvesting components. Additionally, the faster turnover of key polyubiquitinated antioxidant proteins in S11 729-10 likely represents a critical mechanism for protecting against oxidative damage and enhancing tolerance under prolonged heat stress. This is the first time that the application of polyubiquitin-omics has been utilized in turfgrass. These findings provide deeper insights into protein metabolism underlying heat tolerance. Key stress-related traits or proteins identified in this study could be utilized to help develop new cultivars with enhanced tolerance to heat stress.

INSTRUMENT(S):

ORGANISM(S): Agrostis Stolonifera

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Chau-Wen Chou

LAB HEAD: David Jespersen

PROVIDER: PXD060809 | Pride | 2025-10-20

REPOSITORIES: Pride

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			Action	DRS
	QFan_2024-0109_04_4-1.raw	Raw
	QFan_2024-0109_06_4-2.raw	Raw
	QFan_2024-0109_08_4-3.raw	Raw
	QFan_2024-0109_10_4-4.raw	Raw
	QFan_2024-0109_18_4-5.raw	Raw

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Protein Metabolism Underlying Heat Tolerance in Contrasting Creeping Bentgrass Lines: Insights From Gel-Free Proteomics and Polyubiquitin-Omics.

Fan Qianqian Q Chou Chau-Wen CW Jespersen David D

Physiologia plantarum 20250901 5

One of the major disfunctions in heat-stressed plants is enhanced protein damage. Creeping bentgrass (Agrositis stolonifera L.) is an economically important perennial grass species but it is sensitive to high temperatures. Several experimental lines of creeping bentgrass varied in response for physiological traits, total protein content, and protein degradation rates in response to heat stress. The ubiquitin-proteasome system plays a crucial role in the removal of damaged proteins, and there is ...[more]

PMID: 41070926

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