Dataset Information

Receptor kinase pathway signal tuning through a non-transcriptional incoherent feedforward loop

ABSTRACT: This project investigated an auto-regulatory feedback in a plant receptor kinase pathway: The signaling of the endogenous CLAVATA3/EMBRYO SURROUNDING REGION 45 (CLE45) peptide via the receptor kinase BARELY ANY MERISTEM 3 (BAM3) in Arabidopsis (Arabidopsis thaliana) vascular development. Downstream, the plant-specific MEMBRANE-ASSOCIATED KINASE REGULATOR (MAKR) family protein MAKR5 conveys CLE45-BAM3 signaling, which is amplified by CLE45-BAM3-triggered enhancement of MAKR5 production through the stimulation of MAKR5 mRNA translation. At the same time, this signal amplification is dampened through CLE45-stimulated MAKR5 phosphorylation, which inactivates MAKR5, enhances its turnover and impinges on MAKR5 mRNA levels. In summary, the results suggest a non-transcriptional incoherent feedforward loop in which CLE45-BAM3 signaling is amplified via translation of MAKR5 mRNA yet also balanced via phosphorylation of MAKR5 protein.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cotyledon, Seedling, Root

SUBMITTER: Christian Hardtke

LAB HEAD: Christian Hardtke

PROVIDER: PXD061525 | Pride | 2025-05-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	151203-7928-31-Kang.sf3	Other
	151203-7928-31-Kang_mzIdent_export.zip	Other
	160226-8143-46-Kang.sf3	Other
	160226-8143-46-Kang_mzIdent_export.zip	Other
	160429-8404-05-Kang.sf3	Other

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Publications

Receptor kinase pathway signal tuning through a nontranscriptional incoherent feedforward loop.

Wang Qian Q Kang Yeon Hee YH Hardtke Christian S CS

Proceedings of the National Academy of Sciences of the United States of America 20250417 16

Cellular signaling processes can elicit powerful responses and may need to be amplified to be efficient or dampened to prevent overstimulation. Therefore, they often involve autoregulatory feedbacks. Receptor kinase signaling pathways are abundant in plants, where they convey the presence of both exogenous and endogenous ligands. Among them, endogenous CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptide signaling acts in an inherently quantitative manner to determine the size of stem cell pools and ...[more]

PMID: 40244667

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