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Defining the interactome of ARID5B by AviTag protein pull-down coupled to quantitative mass spectrometry.

ABSTRACT: Among the strongest associations with pediatric B-cell acute lymphoblastic leukemia (B-ALL) are risk non-coding variants localized in the intron of ARID5B (AT-rich interaction domain 5B), a poorly characterized DNA-binding protein. In this study, we defined the interactome of ARID5B by LC-MS/MS and identified transcriptional repressors HDAC1, HDAC2, MIER1 and C16ORF87 as interactors. Structural predictions revealed that ARID5B and its interactors assemble into a repressor complex. We further validated and characterized the molecular function of the ARID5B repressor complex in B cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney Cell

SUBMITTER: Fabian Frommelt

LAB HEAD: Davide Seruggia

PROVIDER: PXD061867 | Pride | 2026-05-19

REPOSITORIES: Pride

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			Action	DRS
	2025-02-06-decoys-contam-uniprotkb_AND_reviewed_true_AND_model_o_2025_02_06.fasta.fas	Fasta
	2025_Sample_annotation.txt	Txt
	ARID5B_prot_norm_imputed.txt	Txt
	CC_154_HEK-BirA-1.pepXML	Pepxml
	CC_154_HEK-BirA-1.raw	Raw

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