Dataset Information

Human endosomal proximity proteome

ABSTRACT: Here, using comparative proximity proteomics of critical retrieval sub-domain components Retromer and Retriever, their cargo adaptors, and a component of the opposing ESCRT-degradative sub-domain, we provide a data-rich resource that identifies new molecular details of retrieval sub-domain composition and organization, including an unrecognised complexity in the interface of Retromer with RAB GTPases.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Carlos Anton-Plagaro

LAB HEAD: Peter J. Cullen

PROVIDER: PXD061918 | Pride | 2025-08-04

REPOSITORIES: Pride

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Publications

Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation.

Antón-Plágaro Carlos C Chen Kai-En KE Guo Qian Q Liu Meihan M Evans Ashley J AJ Lewis Philip A PA Heesom Kate J KJ Wilkinson Kevin A KA Collins Brett M BM Cullen Peter J PJ

Nature communications 20250730 1

Endosomal retrieval and recycling of integral cargo proteins is essential for cell and organism development and homeostasis and is orchestrated through a specialised endosomal nanodomain, the retrieval sub-domain. Sub-domain dysfunction is associated with human disease, but our mechanistic understanding of its function remains poorly described. Here, using proximity proteomics of retrieval sub-domain components Retromer and Retriever we identify mechanistic details of retrieval sub-domain compos ...[more]

PMID: 40738907

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