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Analysis of the nuclear proteome fraction of rAAV2-plasmid transfected HEK-293 cells during AAV production

ABSTRACT: In this comparative proteomic analysis, we focused on proteins in the nuclear fraction. As the nucleus is the epicenter of DNA uptake, transcription, capsid assembly and packaging. Three HEK-293 cell samples i) not transfected, ii) MOCK plasmid transfected, and iii) triple-transfected with rAAV2-production plasmids were harvested 24 h and 72 h after transfection. In total 3384 proteins in all samples were identified revealing regulated proteins due to transfection and rAAV production.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Raimund Hoffrogge

LAB HEAD: Prof. Kristian Marc Müller

PROVIDER: PXD062079 | Pride | 2025-07-21

REPOSITORIES: pride

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	2022-03-11_Susi_120min_Probe-10.raw	Raw
	2022-03-11_Susi_120min_Probe-12.raw	Raw
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Publications

Nuclear Fraction Proteome Analyses During rAAV Production of AAV2-Plasmid-Transfected HEK-293 Cells.

Golm Susanne K SK Hoffrogge Raimund R Müller Kristian M KM

International journal of molecular sciences 20250630 13

Recombinant adeno-associated virus (rAAV) is the leading vector for gene replacement therapy; however, the roles and regulation of host proteins in rAAV production remain incompletely understood. In this comparative proteomic analysis, we focused on proteins in the nucleus, the epicenter of DNA uptake, transcription, capsid assembly, and packaging. HEK-293 cells were analyzed under the following three conditions: (i) untransfected, (ii) mock-transfected with the ITR and an unrelated plasmid, and ...[more]

PMID: 40650119

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