Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The RNA pulldown of MEG3 to identify RNA-binding proteins

ABSTRACT: By using biotinylated MEG3 and streptavidin-labeled magnetic beads, identify the RNA-binding proteins of MEG3.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Smooth Muscle Cell

SUBMITTER: Can Wang

LAB HEAD: Yong Ji

PROVIDER: PXD062094 | Pride | 2025-06-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Ingel_Negative_3_chenjiyu_jiyong_20200804.wiff	Wiff
	Ingel_Negative_3_chenjiyu_jiyong_20200804.wiff.scan	Wiff
	Ingel_antisense_2_chenjiyu_jiyong_20200804.wiff	Wiff
	Ingel_antisense_2_chenjiyu_jiyong_20200804.wiff.scan	Wiff
	Ingel_sense_1_chenjiyu_jiyong_20200804.wiff	Wiff

Items per page:

1 - 5 of 7

Similar Datasets

LC-MS by circHIPK3 probe method in human oral submucosal fibroblasts

Project description:To identify proteins to which circHIPK3 may bind and participate in the regulation of PDGF-BB-induced biological behavior in oral mucosal FBs, the circHIPK3 probe was designed for RNA pull-down experiment, and the proteins obtained from RNA pull-down were identified by silver staining, mass spectrometry and bioinformatics (COG, KEGG, PPI) analysis.

2025-05-13 | PXD062842 | Pride

BioID to identify RRas2-binding proteins.

Project description:By using biotin ligase (BirA*) - RRas2 fusion overexpression lentivirus to identify RRas2-binding proteins.

2025-06-30 | PXD062082 | Pride

Single cell RNA-seq and BCR-seq of peritoneal B cells

Project description:B1 cells account for the majority of B cell population in the peritoneal cavity, and are essential for the innate immune responses and maintaining the homeostasis. The origin of the B1 cells and how to form the B1 cells pool in the postnatal life remain unknown. And the heterogeneity of B1 cells can largely affect the functions of B1 cells. Until now, nobody has performed the single cell RNA-seq of peritoneal B cells. In order to reveal the characteristics of peritoneal B cells, we have performed the scRNA-seq and scBCR-seq of the peritoneal B cells of mouse from different stages.

2022-05-21 | E-MTAB-10081 | biostudies-arrayexpress

CSL ChIP-seq

Project description:The expression of a large proportion of the fission yeast genome changes periodically with the cell cycle. Several key transcription factors have been identified that regulate these oscillating and interdependent waves of gene expression. However, for a significant number of cell cycle-regulated genes the regulator(s) driving these oscillations remain unknown. Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell cycle progression, yet the details of their functioning are poorly understood. Using a combination of transcriptome profiling and genome-wide mapping of CSL-DNA interactions we have identified a comprehensive set of CSL-regulated genes. Our data indicate that Cbf11 and Cbf12 contribute directly and indirectly to the regulation of periodically expressed genes in fission yeast. We show that during S phase/cytokinesis Cbf11 directly activates the transcription of several periodic genes required in the cell to prevent catastrophic mitosis. In agreement with these findings, multiple aspects of cell cycle progression are perturbed when CSL cellular levels are genetically manipulated. We have identified Cbf11 as a novel cell cycle phase-specific activator of genes required for proper coordination of cell and nuclear division, and prevention of catastrophic mitosis in fission yeast.

2015-09-18 | E-MTAB-2725 | biostudies-arrayexpress

Sequence Requirements for Cascade Function in a Type I-E CRISPR System: RNA-Seq

Project description:We measured global RNA levels using RNA-seq in cas3 E. coli cells with intact CRISPR arrays or cells with the CRISPR-I array deleted to determine if off-target events driven by endogenous spacers affect local gene expression.

2017-08-24 | E-MTAB-5971 | biostudies-arrayexpress

Sequence Requirements for Cascade Function in a Type I-E CRISPR System: RNA-Seq

Project description:We measured global RNA levels using RNA-seq in cas3+ E. coli cells with intact CRISPR arrays or cells with the CRISPR-I array deleted to determine if off-target events driven by endogenous spacers affect RNA levels.

2018-02-10 | E-MTAB-6446 | biostudies-arrayexpress

Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding

Project description:We investigated the effect that intragenic s54 binding sites have on transcripton elongation. In an rpoN deletion we have over-expressed rpoN from a plasmid (with vector only contol) and then looked at changes in transcription elongation around intragenic s54 binding sites by RNA-seq. We are also looking at changes in transcription initiation/elongation at sites of sigma factor overlap.

2015-08-06 | E-MTAB-3699 | biostudies-arrayexpress

SuhB associates with Nus factors to facilitate 30S ribosome biogenesis in Escherichia coli

Project description:This experiment consists of two studies. The first where two independent biological replicates of MG1655, MG1655 thyA suhB::thyA and MG1655 thyA nusB::thyA were each grown in LB to mid-exponential phase. RNA-seq and associated data analysis were performed as described previously (Stringer et al., 2014. PMID 24272778). The second where five cultures of MG1655 thyA suhB::thyA were grown overnight from single colonies at 37 C in LB. 5 L of each overnight culture was spread on LB agar and incubated at 30 C, the non-permissive temperature for suhB mutants. One suppressor mutant colony was selected from each plate. rnc was PCR amplified from colonies using oligonucleotides JW836-JW837, and the PCR products were sequenced to identify the presence, if any, of suppressor mutations. Genomic DNA from a strain with wild-type rnc was prepared using a DNeasy Blood and Tissue Kit (Qiagen). A DNA library was prepared using a Nextera kit (Illumina). The library was sequenced (paired-end reads) using an Illumina MiSeq instrument.

2016-01-20 | E-MTAB-4240 | biostudies-arrayexpress

The evolutionary impact of Intragenic FliA Promoters

Project description:We compared RNA levels in wild-type Salmonella enterica serovar Typhimurium with RNA levels in a mutant strain in which the fliA gene was deleted.

2017-09-18 | E-MTAB-6048 | biostudies-arrayexpress

RNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)

Project description:We used RNA-seq to determine differences in RNA levels between wild-type E. coli K-12 and a ΔphoB mutant, each grown in MOPS media with low phosphate (0.2 mM).

2020-07-12 | E-MTAB-9292 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data