An Essential Factor for Apicoplast Fission and Inheritance in Malaria Parasites

ABSTRACT: Blood-stage Plasmodium falciparum parasites rely on a non-photosynthetic plastid, the apicoplast, for survival, making it an attractive target for antimalarial intervention. Like the mitochondrion, the apicoplast cannot be generated de novo and must be inherited by daughter parasites during cell division. This process relies on coordinated fission events, yet the molecular mechanisms remain poorly understood. Here, we identify a previously uncharacterized P. falciparum protein (Pf3D7_0613600), which we name PfAnchor, as a key regulator of apicoplast fission. Using Ultrastructure Expansion Microscopy (U-ExM), we show that PfAnchor localizes to the apicoplast throughout the asexual life cycle. Conditional depletion disrupts apicoplast fission, leading to incomplete cytokinesis and parasite death. Notably, loss of apicoplast branching via azithromycin treatment rescues these defects, underscoring Anchor’s specific role in apicoplast division. Immunoprecipitation further identifies an interaction with the dynamin-like GTPase PfDyn2, a key mediator of both apicoplast and mitochondrial fission, establishing PfAnchor as the first apicoplast-specific dynamin adaptor protein. Our findings define PfAnchor as an essential factor for apicoplast fission and inheritance in P. falciparum blood-stage parasites, highlighting parasite-specific organelle division as a potential vulnerability for therapeutic intervention.

INSTRUMENT(S):

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

SUBMITTER: Emma Doud  

LAB HEAD: Amber Mosley

PROVIDER: PXD062126 | Pride | 2025-10-27

REPOSITORIES: Pride