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Translational repression by 4E-T is crucial to maintain the prophase-I arrest in vertebrate oocytes

ABSTRACT: Meiotic maturation of vertebrate oocytes occurs in the near-absence of transcription. Thus, female fertility relies on timely translational activation of maternal transcripts stockpiled in full-grown prophase-I-arrested oocytes. However, how expression of these mRNAs is suppressed to maintain the long-lasting prophase-I arrest remains mysterious. Utilizing fast-acting TRIM-Away, we demonstrate that acute loss of the translation repressor 4E-T triggers spontaneous release from prophase-I arrest in mouse and frog oocytes. This is due to untimely expression of key meiotic drivers like c-Mos and cyclin-B1. Notably, mutant 4E-T associated with premature ovarian insufficiency fails to maintain the prophase-I arrest in Xenopus oocytes. We further show that 4E-T association with eIF4E and PATL2 is critical for target mRNA binding and repression. Thus, 4E-T is a central factor in translational repression of mRNAs stockpiled in full-grown oocytes for later activation and, therefore, essential to sustain the oocyte pool throughout the reproductive lifespan of female vertebrates.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Oocyte

SUBMITTER: Florian Stengel

LAB HEAD: Florian Stengel

PROVIDER: PXD062261 | Pride | 2025-07-18

REPOSITORIES: Pride

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			Action	DRS
	DIA_LFQ_values_and_processed_data.xlsx	Xlsx
	E2501_orthj_346.raw	Raw
	E2501_orthj_347.raw	Raw
	E2501_orthj_348.raw	Raw
	E2501_orthj_351.raw	Raw

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