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TBK1 phosphorylates NAP1 on several sites

ABSTRACT: NAP1 is known to be phosphorylated on several sites by TBK1. We decided to identify the phosphorylation sites on NAP1. For this, using an anti-GFP antibody, we immunoprecipitated a version of NAP1 fused to the GFP protein at its amino terminus (GFP-NAP1) from HEK293T cells co-expressing GFP-NAP1 and TBK1 fused to the mCherry protein at its amino terminus (mC-TBK1). Immunoprecipitates were analyzed by SDS-PAGE and WB, and the bands corresponding to NAP1 were cut and digested by trypsin protease before analysis by LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Laïla sago

LAB HEAD: Yves Gaudin

PROVIDER: PXD062298 | Pride | 2026-07-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F081857.mgf	Mgf
	F081857.mzid	Mzid
	F081951.mgf	Mgf
	F081951.mzid	Mzid
	PhosNAP1_220424.fasta	Fasta

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