Dataset Information

Specimen Inactivation Methods for Proteomics – Comparisons of irradiation, chemical, and heat treatments on downstream serum analyses

ABSTRACT: Numerous studies have described the efficacy of heat inactivation, gamma irradiation, or treatment with guanidium-based chaotropic salts (e.g. TRIzol®) for pathogen inactivation of biological samples to ensure biosafety and biosecurity. However, the effect of these methods on the greater serum proteome are less studied. Here we sought to comprehensively measure the effects of three routinely used pathogen inactivation methods on the serum proteome of Rhesus macaques by characterizing the serum proteome pre-and-post inactivation treatment. Using data independent aquisition-based shotgun LCMS/MS, we evaluated total peptide/protein detection and individual protein abundances (e.g. ALB, APOA1, and CRP) across inactivation methods and compared to their untreated controls. Specifically, we observed improved quantitative reproducibility in gamma-irradiated samples across biological, technical, and experimental replicates compared to chemical inactivation and different heat combinations. These findings represent the first direct, experimental comparisons of effective pathogen inactivation methods on the serologic profiles of non-human primates and provide useful criteria for evaluating methods for biological specimen inactivation prior to proteomic analysis.

INSTRUMENT(S):

ORGANISM(S): Macaca Mulatta (rhesus Macaque)

TISSUE(S): Blood Serum

SUBMITTER: Brooke Genovese

LAB HEAD: Brian Bird

PROVIDER: PXD062367 | Pride | 2025-10-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20250328_161211_SpNd_set1.sne	Other
	20250328_162316_SpNd_set2.sne	Other
	FL130923_2setGenov75MaqSer_DiaW46-90m_1A.raw	Raw
	FL130923_2setGenov75MaqSer_DiaW46-90m_1F.raw	Raw
	FL130923_2setGenov75MaqSer_DiaW46-90m_1K.raw	Raw

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Publications

Specimen Inactivation Methods for Proteomics─Comparisons of Irradiation, Chemical, and Heat Treatments on Downstream Serum Analyses.

Genovese Brooke N BN Randhawa Nistara N Grigorean Gabriela G Drazenovich Tracy T Phinney Brett S BS Van Rompay Koen K A KKA Yee JoAnn J Mazet Jonna A K JAK Bird Brian H BH

Journal of proteome research 20250919 10

Multiomic techniques, including proteomics, can provide novel insights for both pathogen detection and assessment of host responses to infection. Numerous studies have described the efficacy of heat inactivation, γ irradiation, or treatment with guanidinium-based chaotropic salts (e.g., TRIzol) for pathogen inactivation of biological samples to ensure biosafety and biosecurity. However, the effect of these methods on the greater serum proteome is less studied. Here, we sought to comprehensively ...[more]

PMID: 40969138

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