Dataset Information

Characteristics of tendinopathy development in humans

ABSTRACT: Overuse injuries of tendon (tendinopathy) represents a significant musculoskeletal health problem, but its pathogenesis is only sparsely understood. We investigated the characteristics behind the development of tendinopathy in humans that had either early phase (1-2 months of symptoms, n=14) (ET) or more chronic (> 3 months, n=16) (CT) patella tendinopathy, vs healthy subjects (n=15) (CTRL). We found that pain symptoms rose and function declined with duration of injury, and that it was correlated to tendon size measured with MRI (3T and 7T). Tendon blood flow (US Doppler) increased gradually over the duration of tendinopathy, whereas peritendinous flow only rose in CT. Histological mapping (traditional multiplex and Cell Dive) of tendon vasculature showed marked changes only in CT, indicating that early in tendinopathy flow is increased in existing vessels whereas angiogenesis (both rise in vessel size and density) is a late phenomenon, and that tendinopathy leads to perivascular cell re-cruitment and expansion of lymphatic vessels. Further, matrisome analysis (proteomics) re-vealed that most matrix protein regulation occurs rather late in tendinopathy. It is concluded that the early phase of tendinopathy is dominated by pain symptoms that are correlated with tendon swelling together with increased blood flow due to flow regulation, whereas the more chronic state of tendinopathy is characterized by angiogenesis, and protein changes in the matrisome.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tendon

SUBMITTER: Luisa Marie Schmidt

LAB HEAD: Michael Kjaer

PROVIDER: PXD062414 | Pride | 2026-03-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BBH148_BLANK_GH12_4-14-2023_16-57-46_9866.d.zip	Other
	BBH148_BLANK_GH12_4-15-2023_12-54-51_9886.d.zip	Other
	BBH148_BLANK_GH12_4-16-2023_08-51-58_9906.d.zip	Other
	BBH148_BLANK_GH12_4-16-2023_23-42-24_9921.d.zip	Other
	BBH148_Hela_06_BA4_4-14-2023_15-56-24_9865.d.zip	Other

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Publications

Increased Blood Flow and Tendon Swelling Precedes Vascular Expansion and Tissue Matrix Changes In Early Human Tendinopathy: A Potential Window for Superior Treatment Response.

Merkel Max F R MFR Malmgaard-Clausen Nikolaj M NM Lendal Marius M Siebner Hartwig R HR Svensson René B RB Dakin Stephanie G SG Krüger Marcus M Schmidt Luisa L Agergaard Jakob J Yeung Ching-Yan Chloé CC Magnusson S Peter SP Kjaer Michael M

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20251212

Tendinopathy represents a major musculoskeletal health problem, yet its pathogenesis remains poorly understood. Tendinopathy development is studied in humans with early (< 3 months of symptoms, n = 14) (ET) or chronic (> 3 months, n = 16) (CT) patellar tendinopathy and in healthy subjects (n = 15) (CTRL). Pain increases, and function declines with tendinopathy duration and correlated with tendon size (3T and 7T MRI). Tendon blood flow (Doppler ultrasonography) increases gradually in ET and CT, w ...[more]

PMID: 41388235

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