Dataset Information

Identification and Characterisation of Taxonomically Informative Peptides

ABSTRACT: Hair fibers serve as a prevalent and valuable form of biological trace evidence. Taxonomic classification of hair recovered during a criminal investigation provides crucial forensic intelligence and aids in evidence prioritisation. Traditional classification methods rely heavily on the analysis of hair shaft morphology, which requires individual analysis, can have limited taxonomic resolution, and is difficult to scale. Mass spectrometry-based proteomics can provide a biomolecular alternative by use of taxonomic amino acid polymorphisms to resolve the species of origin of trace evidence. In this study a proteomics workflow was developed for the discovery and characterization of taxonomically diagnostic hair peptides. Taxa were selected with a focus on relevance to crime scene investigation. A panel of 226 taxonomically informative peptides were identified and characterized, 209 of which are novel to this study. The biomarker panel includes 59 peptides with apparent single-species resolution, with remaining biomarkers being diagnostic in combination. The study also included 3 positive control keratin sequences where at least 2 are documented to occur in all mammals. The panel of taxa-discriminating markers was subsequently integrated into a forensic workflow, optimized for universal sample preparation and standardised LC/MS/MS data acquisition. The developed workflow was used to analyze single fur-hair shafts (20 mm), from four individuals from each species of interest. The data was further processed using a curated protein database, an efficient and validated bioinformatics workflow. The method was able to classify all hairs analysed with genus or species level resolution without prior source information.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat) Equus Caballus (horse) Felis Catus (cat) (felis Silvestris Catus) Camelus Dromedarius (dromedary) (arabian Camel) Vulpes Vulpes (red Fox) Sus Scrofa Domesticus (domestic Pig) Canis Familiaris (dog) (canis Lupus Familiaris) Vicugna Pacos Bos Taurus (bovine) Homo Sapiens (human) Ovis Aries Oryctolagus Cuniculus (rabbit) Capra Hircus (goat) Cavia Porcellus (guinea Pig) Mus Musculus (mouse)

TISSUE(S): Hair Shaft

SUBMITTER: Romy Keane

LAB HEAD: Joel Gummer

PROVIDER: PXD062555 | Pride | 2026-05-13

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2023_07_24_012_PVP0010A_13_6.mgf	Mgf
	2023_07_24_012_PVP0010A_13_6.raw	Raw
	2023_07_24_013_PVP0010B_13_6.mgf	Mgf
	2023_07_24_013_PVP0010B_13_6.raw	Raw
	2023_07_24_016_PVP0011A_19_6_20230725163823.mgf	Mgf

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Publications

Taxonomic Source Identification of Hair.

Keane Romy E RE Gummer Joel P A JPA Tidy Rebecca J RJ Parker Glendon J GJ

Journal of proteome research 20260505

Hair fibers are a common form of biological trace evidence. Taxonomic classification can provide valuable forensic intelligence and aid in evidence prioritization. Traditional methods of classification, based on hair morphology, require expert interpretation, offer limited taxonomic resolution, and are not easily scalable. Mass-spectrometry-based proteomics provides a biomolecular alternative through taxon-specific amino acid polymorphisms to resolve species identification. In this study, a prot ...[more]

PMID: 42085601

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