Proteomics

Dataset Information

0

Proteomic Profiling Of Bladder Cancer Patients-Derived Extracellular Vesicles

ABSTRACT: Extracellular vesicle (EV) proteomics holds great promise for non-invasive cancer biomarker discovery. This study presents the first comprehensive proteomic characterization of EVs from tissue, plasma, and urine, focusing on bladder cancer BlCa. EVs were isolated via ultracentrifugation, and their cargo was analyzed, comparing BlCa patients to controls and patients with clear cell renal cell carcinoma (ccRCC) and prostate cancer (PCa), to identify BlCa-specific markers. Urine proved to be a rich source of BlCa EVs, enabling tissue-to-urine molecular tracking and insights into BlCa communication. Differential proteomic analysis revealed distinct BlCa EV profiles compared to healthy donors (HDs), ccRCC, and PCa. Importantly, we identified several candidate biomarkers, including ADAM8 and MMP12, exclusively present in BlCa tissue (50% and 64%, respectively) and urine EVs (83% each). Furthermore, six BlCa urinary EV proteins – ACKR1 (66%) and DHCR7, ERMAP, PTGS2, UBXN4, and GYPC (50% each) – were absent in HDs, ccRCC, and PCa. This study identifies several BlCa-derived EV proteins with strong potential for the development of non-invasive EV-based diagnostics and monitoring strategies for BlCa, potentially contributing to advancing precision medicine approaches for this malignancy .

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine, Prostate Gland, Blood Plasma, Extracellular Vesicle, Bladder, Kidney

DISEASE(S): Urinary Bladder Cancer

SUBMITTER: Hugo Osorio  

LAB HEAD: Carmen Jerónimo

PROVIDER: PXD062618 | Pride | 2026-06-08

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
BlCa_Plasma11_T_CL15.raw Raw
BlCa_Plasma16_T_CL16.raw Raw
BlCa_Plasma17_T_CL17.raw Raw
BlCa_Plasma19_T_CL18.raw Raw
BlCa_Plasma1_T_CL13.raw Raw
Items per page:
1 - 5 of 49
altmetric image

Publications

From cell lines to the clinic: identifying a urinary BlCa-EV signature through comparative proteomics of bladder cancer lysates and extracellular vesicles.

Lourenço Catarina C   Constâncio Vera V   Tavares Nuno Tiago NT   Monteiro-Reis Sara S   Silva-Santos Rui R   Lobo João J   Carvalho Ângela    Jerónimo Carmen C  

Cell communication and signaling : CCS 20260409 1

<h4>Background</h4>There is an urgent need for the development of innovative diagnostic and therapeutic strategies in bladder cancer (BlCa) management, due to its significant incidence, mortality, and morbidity. Extracellular vesicles (EVs) constitute promising candidates as biomarkers, as their cargo is highly protected from degradation, and they can be isolated non-invasively from urine. Furthermore, they play a pivotal role in intercellular communication that drives disease progression, makin  ...[more]

Similar Datasets

Proteomics From Cell Lines To The Clinic: Identifying A Specific Urinary BlCa-EV Signature In Comparative Proteomics Of Bladder Cancer Lysates And Extracellular Vesicles
2026-06-08 | PXD071837 | Pride
Transcriptomics Urinary Extracellular Vesicles RNA Profile in Clear Cell Renal Cell Carcinoma as Biomarker
2016-09-01 | E-GEOD-72922 | biostudies-arrayexpress
Transcriptomics Effects of urinary extracellular vesicles from prostate cancer patients on the transcriptomes of cancer-associated and normal fibroblasts
2023-04-01 | E-MTAB-11022 | biostudies-arrayexpress
Transcriptomics Effects of prostate cancer patient urinary extracellular vesicles and hypoxic extracellular vesicles from prostate cancer cell lines on the transcriptomes of cancer-associated and normal fibroblasts
2023-04-01 | E-MTAB-11317 | biostudies-arrayexpress
Transcriptomics Proteomic signatures of extracellular vesicles secreted by non-mineralizing and mineralizing human osteoblasts and stimulation of tumor cell growth
2014-10-28 | E-GEOD-56172 | biostudies-arrayexpress
Transcriptomics The target gene investigation in osteoblats (OBs) treated with mmu-miR-1963 enriched in EVs from OCs with prostate cancer (PCa) cells [target_EV-miR-1963]
2024-06-06 | GSE268549 | GEO
Transcriptomics The target gene investigation in osteoclats (OCs) treated with mmu-miR-5112 enriched in EVs from OCs with prostate cancer (PCa) cells [target_EV-miR-5112]
2024-06-06 | GSE268550 | GEO
Transcriptomics miR-26a regulate the extracellular vesicle secretion from prostate cancer cells via targeting PFDN4, CHORDC1 and SHC4
2020-05-12 | GSE132161 | GEO
Genomics Cell and microvesicle urine microRNA deep sequencing profiles from healthy individuals
2016-01-28 | GSE72183 | GEO
Transcriptomics Urinary Extracellular Vesicles RNA Profile in Clear Cell Renal Cell Carcinoma as Biomarker
2016-09-01 | GSE72922 | GEO