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Proximity labelling of the post-Golgi AP complexes in Plasmodium falciparum

ABSTRACT: Adaptor protein (AP) complexes are pivotal components of the post-Golgi trafficking and endocytosis process and are involved in the selection, package and sorting of cargo into vesicles to different destination compartments in eukaryotic cells. The main objective of this project was to investigate the proxiome of the post-Golgi adaptors AP-1, AP-3 and AP-4 in the human malaria parasite Plasmodium falciparum, using the DiQ-BioID approach. To this end, malaria parasites were genetically modified via selection linked integration (SLI) to express endogenously 2xFKBR-GFP-2xFKBP-tagged versions of the μ subunits of AP-1, AP-3 and AP-4 complexes, followed by transfections with plasmids to episomally express miniTurbo-FRB-mCherry (N-terminal) and mCherry-FRB-miniTurbo (C-terminal). The conditional recruitment of the miniTurbo to AP foci (trans-Golgi localisation) was induced by addition of rapalog. Consequently, a mass spectrometry analysis of biotinylated proteins resulted in a list of high-confidence hits for each adaptor, which identified potential interactors and compartment neighbours. The resulting data provides an unconventional insight into the vesicle trafficking and cargo sorting machinery in malaria parasites.

INSTRUMENT(S):

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

SUBMITTER: Gala Ramon-Zamorano

LAB HEAD: Tobias Spielmann

PROVIDER: PXD062862 | Pride | 2025-09-17

REPOSITORIES: Pride

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			Action	DRS
	20221110_Exploris1_SA_GRZ_AP1_miniT_Ct_Fw.raw	Raw
	20221110_Exploris1_SA_GRZ_AP1_miniT_Ct_Rv.raw	Raw
	20221110_Exploris1_SA_GRZ_AP1_miniT_Nt_Fw.raw	Raw
	20221110_Exploris1_SA_GRZ_AP1_miniT_Nt_Rv.raw	Raw
	20231101_Exploris1_SA_GZ_TSext_malaria_AP3_cterm_GFP_dimethyl_10_R.raw	Raw

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