Dataset Information

HUMAN PLASMAS FROM ELDERLY PEOPLE DISPLAY A PROTEIN SIGNATURE SUGGESTIVE OF A CHRONIC INFLAMMATORY STATE

ABSTRACT: Blood from elderly individuals exhibits a reduced "rejuvenating" effect compared to that of young donors, and plasma from older subjects used as a supplement in cell culture media is less effective than plasma from younger individuals. A mass spectrometry-based proteomic analysis of plasma samples from 229 blood donors—including a prepubertal group, a healthy young adult group, and a cohort of individuals over 75 years old—revealed a chronic inflammatory state in the elderly population, along with complement activation and negative regulation of blood coagulation. This inflammatory condition was confirmed by elevated levels of classical pro-inflammatory cytokines in the plasma of older individuals, as measured by microarray and Milliplex Luminex assays. Moreover, the elderly group showed a reduced production of antibody light chains, suggesting concurrent immunosenescence. We identified 25 proteins whose increased abundance, together with acquired immune aging, may constitute a plasma proteomic signature of aging. The degree of upregulation of these proteins varied among elderly subgroups with different lifestyles. An evaluation of the expression patterns of the 25 consistently elevated proteins revealed distinct protein clusters with differential expression across three lifestyle-based subgroups. Notably, a lifestyle aimed at preserving good physical and/or cognitive function appeared to attenuate the intensity of the aging-related proteomic signature. To explore potential gender-related differences in plasma protein expression, we compared samples from male and female donors. Among elderly individuals, no major differences were observed, except for an increased level of Pregnancy Zone Protein (PZP) in females. In contrast, several gender-specific differences were identified in the plasma profiles of young donors.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Martina Bartolucci

LAB HEAD: Andrea Petretto

PROVIDER: PXD063248 | Pride | 2026-02-02

REPOSITORIES: Pride

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Exploring the impact of age, sex and life experiences on plasma inflammatory profiles through comparative proteomics.

Bartolucci Martina M Utyro Olga O Muraglia Anita A Repetto Alessia A Agostini Vanessa V Pizzonia Monica M Ottaviani Silvia S Tripodi Gino G Filaci Gilberto G Cancedda Ranieri R Petretto Andrea A Mastrogiacomo Maddalena M

Frontiers in immunology 20260107

<h4>Background</h4>In the heterochronic parabiosis model it has been shown that blood from elderly animals exhibits markedly reduced rejuvenating effects compared to that of young organisms. Furthermore, human plasma from older subjects, when used as a supplement in cell culture media, is significantly less effective than plasma derived from younger individuals. This study analyzed plasma from a cohort of 229 subjects by a proteomic approach to reveal age-related changes.<h4>Methods</h4>A mass s ...[more]

PMID: 41573543

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Project description:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. </p> Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Plasma and RBC data from all 30 subjects can be found under MTBLS266 and MTBLS267, respectively.

			Action	DRS
	20240620_084328_Mastrogiacomo_age.sne	Other
	Age_Plasma.zip	Other
	Ctr10_140.raw	Raw
	Ctr11_141.raw	Raw
	Ctr12_142.raw	Raw