Dataset Information

Stress specific SCE1-interactome characterised by IP-MS analysis

ABSTRACT: The AtSCE1-mVenus was immunoprecipitated using anti-GFP magnetic beads from Arbidopsis root cultures upon control, salt, mannitol and flg22 treatments. As a negative control, root tissues from pMDS1-VAM3 and pMDS1-SHR were utilized to account for non-specific binding to GFP. The eluate sapmles were digested using trypsin/Lys-C in gel and further LC-MS/MS analysis was performed using Thermo Fisher Q Exactive Mass Spectrometer.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Root

SUBMITTER: Kishor D Ingole

LAB HEAD: Kathryn S. Lilley

PROVIDER: PXD063447 | Pride | 2025-09-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	P1784_P1833_280425.mzid.gz	Mzid
	P1784_P1833_280425.mzid.gz_KI-104__pMDS1_SCE1_Control_1__antiGFP__F024080_.MGF	Mzid
	P1784_P1833_280425.mzid.gz_KI-105__pMDS1_SCE1_Control_2__antiGFP__F024081_.MGF	Mzid
	P1784_P1833_280425.mzid.gz_KI-106__pMDS1_SCE1_Control_3__antiGFP__F024082_.MGF	Mzid
	P1784_P1833_280425.mzid.gz_KI-110__pMDS1_SCE1_NaCl_1__antiGFP__F024083_.MGF	Mzid

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Publications

Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote.

Banda Jason J Ghosh Shraboni S Roy Dipan D Ingole Kishor D KD Clark Lisa L Sharma Eshan E Kakkunath Sumesh S Sue-Ob Kawinnat K Bhosale Rahul R Band Leah L Ghosh Srayan S Wells Darren D Atkinson Jonathan J Provart Nicholas J NJ Bennett Malcolm J MJ Lilley Kathryn S KS Jones Andrew A De Lucas Miguel M Bishopp Anthony A Sadanandom Ari A

Science advances 20250827 35

SUMOylation is essential in plant and animal cells, but it remains unknown how small ubiquitin-like modifier (SUMO) components act in concert to modify specific targets in response to environmental stresses. In this study, we characterize every SUMO component in the <i>Arabidopsis</i> root to create a complete SUMO Cell Atlas in eukaryotes. This unique resource reveals wide spatial variation, where SUMO proteins and proteases have subfunctionalized in both their expression and subcellular locali ...[more]

PMID: 40864707

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Project description:Hypersensitive response-related programmed cell death (PCD) has been extensively analyzed in various plant–virus interactions. However, little is known about changes in gene expression associated with cell death caused by compatible viruses. The synergistic interaction of Potato virus X (PVX) with Plum pox virus (PPV) results in increased symptoms that lead to systemic necrosis (SN) in Nicotiana benthamiana. Here, we performed three transcriptome comparisons in response to i) a PVX recombinant virus expressing the helper component-proteinase (HC-Pro) gene from PPV that leads to SN, ii) a systemic incompatible interaction conferred by the Tobacco mosaic virus (TMV)-resistance gene N (SHR), and iii) the depletion of the PBE subunit of the proteasome that leads to PCD by virus induced gene silencing (VIGS Prot), at early and late stages of infection. Our analysis indicated that the SN response was clustered with SHR by the similarity of their overall gene expression profiles. However, the expression profiles of defence-related and hormone-responsive genes in response to SN were more closely related to the response to VIGS Prot than to that elicited by SHR. This suggests the potential contribution of proteasome dysfunction to the increase in pathogenicity observed in PVX-potyvirus infections We compare the gene expression profiles of Nicotiana benthamiana plants infected with either necrosis-inducing viruses or non-necrosis-inducing viruses, as follow: PVX/HCWT-infected plants versus plants infected with a PVX recombinant virus expressing a PPV HC-Pro mutant (PVX/HCLH) that was unable to induce the SN response (SN comparison), at 7 and 11 days postinoculation (dpi), (ii) TRV:NbPBE-silenced plants versus plants infected with the TRV empty vector (VIGS Prot comparison), at 4 and 8 days after infiltration (dpa), and (iii) TMV-GFP-infected, N-transgenic plants versus wild-type plants infected with TMV-GFP (SHR comparison), at 24 and 72 hours after temperature shift (hts). Per time and treatment, three independent biological replicates were used to monitor differences in gene expression between treatments