Dataset Information

Discovery, characterization and quantification of bioactive peptides contained in Palbio porcine intestinal mucosa hy-drolysate products.

ABSTRACT: Porcine intestinal mucosa hydrolysates (PIMH) are a by-product of heparin production obtained through a specific enzymatic hydrolysis process which can theoretically generate bioactive pep-tides (BAPs). This study aimed to identify, characterize and quantify BAPs in two Palbio products manufactured by Bioiberica S.A.U. (Palafolls, Spain) which are PIMH protein sources used for animal feed: Palbio® HP (PHP) and Palbio® 62 SP® (P62). Using mass spectrometry (MS)-based peptidomics, we analyzed three samples from each product, fractionated based on molecular weight (<3 kDa, 3 to 10 kDa, and >10 kDa). The <3 kDa fraction was analyzed directly, while the other two fractions were enzymatically digested before MS analysis. The workflow identified 961 peptides in PHP and 1134 in P62. Subsequent bioinformatic analysis using public databases (APD2, StraPep, AHTPDB, and BIOPEP-UWM) led to the identification of six significant BAPs in both PHP and P62, with respective quantified amounts (pg peptide/μg sample): DAVEDLESVGK (0.1626, 0.1939), EGIPPDQQRLIFAGK (0.2637, 0.1852), TITLEVEPSDTIENVK (0.3594, 0.4327), TNVPRASVPDGFLS (1.4596, 0.1898), TNVPRASVPDGFLSEL (8.0500, 0.9224), and VHVVPDQLMAF (0.0310, 0.0054). The first three BAPs are related to antimicrobial activity, while the latter three are associated with cytokine/growth factor-like, antioxidant, and immunomodulatory activities. These bioactivities align with previously reported in vivo benefits observed in animal nutrition using Palbio products. Our findings demonstrate that PHP and P62 are valuable sources of BAPs, supporting their potential role in improving animal health and performance.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa (pig)

TISSUE(S): Small Intestine Mucosa

SUBMITTER: Joan Josep Bech-Serra

LAB HEAD: Sergi Segarra

PROVIDER: PXD063531 | Pride | 2025-08-04

REPOSITORIES: Pride

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Publications

The Discovery, Characterization, and Quantification of Bioactive Peptides Contained in Palbio Porcine Intestinal Mucosa Hydrolysate Products.

Segarra Sergi S de la Torre Carolina C Bech-Serra Joan Josep JJ Cucurull Bernat B Marazuela-Duque Anna A Vaquero Alejandro A Martínez-Puig Daniel D Velasco-Alvarez Javier J

International journal of molecular sciences 20250711 14

Porcine intestinal mucosa hydrolysates (PIMHs) are by-products of heparin production obtained through a specific enzymatic hydrolysis process, which can theoretically generate bioactive peptides (BAPs). This study aimed to identify, characterize, and quantify BAPs in two Palbio products manufactured by Bioiberica S.A.U. (Palafolls, Spain), which are PIMH protein sources used for animal feed: Palbio® HP (PHP) and Palbio® 62 SP® (P62). Using mass spectrometry (MS)- ...[more]

PMID: 40724906

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	3842_CT_BSAU003_10_M.raw	Raw
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