Quantitative Proteomic Analysis of Extracellular Vesicles Derived from γ-Irradiated Normal Human Breast Fibroblasts

ABSTRACT: Small extracellular vesicles (EVs) were isolated from a model of normal breast fibroblasts— immortalized cells derived from healthy reduction mammoplasty tissue—treated with γ-radiation to study the effects of radiation therapy on intercellular communication within the breast stroma. EVs were isolated using the gold-standard differential speed centrifugation protocol. Proteins were subsequently extracted, and a quantitative examination of the alterations in the EV proteome was pursued. Both label-free and isobaric label-based methodologies were conducted to comprehensively characterize proteomic changes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast Of Breast, Breast

SUBMITTER: Kristie Lindsey Rose  

LAB HEAD: Kristie Lindsey Rose

PROVIDER: PXD063788 | Pride | 2026-03-02

REPOSITORIES: Pride