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Copper Trafficking in Sheep: A Pilot Proteomic Analysis of Enterocytes and Hepatocytes Under Dietary Copper Supplementation

ABSTRACT: Seven Rambouillet-crossbred wethers were utilized to examine the impact of copper (Cu) supplementation on plasma, bile, liver, duodenum, jejunum, and ileum tissue Cu concentrations and Cu trafficking proteins abundance in the duodenum and liver. Wethers were blocked by body weight and assigned to one of two treatments: 1) control: no supplemental Cu and 2) +Cu: 12 mg of supplemental Cu/kg DM. After receiving treatments for 29 d, all wethers were transported to a USDA-inspected abattoir and harvested. Following harvest, blood, bile, liver, duodenum, jejunum, and ileum samples were collected for Cu concentration analysis. Additional duodenum and liver samples were collected for proteomic analysis.

INSTRUMENT(S):

ORGANISM(S): Ovis Aries

TISSUE(S): Hepatocyte, Duodenum, Liver, Enterocyte Of Epithelium Proper Of Duodenum

DISEASE(S): Disease Free

SUBMITTER: Huey Yi Loh

LAB HEAD: Terry Engle

PROVIDER: PXD063823 | Pride | 2026-05-13

REPOSITORIES: Pride

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			Action	DRS
	HYL4661_MC_Duodenum.mzTab	Mztab
	HYL4661_MC_Liver.mzTab	Mztab
	HYL4661_S1.raw	Raw
	HYL4661_S10.raw	Raw
	HYL4661_S11.raw	Raw

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