Dataset Information

The BRD4-nucleosome interaction is enhanced modestly and non-selectively by histone acetylation

ABSTRACT: BRD4 regulates gene transcription in complex eukaryotes, in part through the binding of its tandem bromodomains to acetylated lysine residues found in histones and transcription factors. Despite pharmacological inhibition of these binding events showing significant promise in preclinical studies, clinical trial data have been less encouraging so far. A stronger understanding of BRD4 biochemistry could provide a route to better outcomes. To advance on prior work, which has focused almost entirely on the binding properties of isolated bromodomains and acetylated peptides, we have sought the preferred nucleosomal binding partner of full-length BRD4. Here we performed affinity pulldowns of HEK293 mononucleosomes against BRD4 to ascertain binding preferences in the nucleosomal context.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jason Low

LAB HEAD: Joel Mackay

PROVIDER: PXD064221 | Pride | 2026-01-12

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	BRD4NucH3K27K36K37Rep1.sky.zip	Other
	BRD4NucH3K27K36K37Rep2.sky.zip	Other
	BRD4NucH3K4Rep2.sky.zip	Other
	BRD4NucH3K9K23rep1.sky.zip	Other
	BRD4NucH3Rep2H3K9K23.sky.zip	Other

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Publications

The BRD4-nucleosome interaction is enhanced modestly and non-selectively by histone acetylation.

Lambrechts Lucien S LS Reid Xavier J XJ Kambanis Lucas L Luong Clement C Kobakhidze Erekle E Daners Andrea A Zhong Yichen Y Patel Karishma K Franck Charlotte K CK Sani Hakimeh Moghaddas HM Taylor Cameron C Low Jason K K JKK Payne Richard J RJ Mackay Joel P JP

Nucleic acids research 20251101 22

BRD4 regulates gene transcription in complex eukaryotes, in part through the binding of its tandem bromodomains to acetylated lysine residues found in histones and transcription factors. Despite pharmacological inhibition of these domains showing promise in preclinical studies, clinical trial data have been less encouraging so far. A stronger understanding of BRD4 biochemistry could provide a route to better outcomes. To advance on prior work, which has focused almost entirely on the binding of ...[more]

PMID: 41459750

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