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Untargeted NoPro-clipping from untramsformed and Hoil-1L over-expressing Huh7 cell lines

ABSTRACT: NoPro-clipping is a method for detecting and quantifying non-proteinaceous ubiquitination from cell lysates and mammalian tissue. The method utilising Ub-clippases to cleave ubiquitin but leave the C-terminal diglycine remnant attached to the protein and non-protein substrates. Following protein exclusion, small molecule diglycine species are selectively labelled with an LPXTGG-containing peptide via the enzyme sortase. While the method can be employed in a targeted workflow to quantify a pre-defined non-protein ubiquitin species, the method can also be employed in an untargeted approach. This submission includes the necessary controls and raw files to identify non-protienaceous ubiquitination present in Huh7 cells either untransformed or over-expressing the E3 ligase HOIL-1L fused to a di-ubiquitin (for increased activity).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte

SUBMITTER: Simon Cobbold

LAB HEAD: Simon Cobbold

PROVIDER: PXD064299 | Pride | 2026-07-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20240614_MJ_2ul_of_400ul_2xUbHOIL1_emptyV_Jopro_Peptide_1.raw	Raw
	20240614_MJ_2ul_of_400ul_2xUbHOIL1_emptyV_Jopro_Peptide_2.raw	Raw
	20240614_MJ_2ul_of_400ul_2xUbHOIL1_emptyV_Jopro_Peptide_3.raw	Raw
	20240614_MJ_2ul_of_400ul_2xUbHOIL1_emptyV_Jopro_noPeptide_1.raw	Raw
	20240614_MJ_2ul_of_400ul_2xUbHOIL1_emptyV_Jopro_noPeptide_2.raw	Raw

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