Dataset Information

CacyBP/SIP - RPL6 interaction: potential influence on ribosome function

ABSTRACT: Previously, we have shown that CacyBP/SIP interacts with NPM1, a protein involved in ribosome biogenesis. In this work, examined the potential impact of CacyBP/SIP on ribosome biogenesis and/or function. At first, we searched for ribosomal proteins (RPs) that might interact with CacyBP/SIP. Using mass spectrometry analysis, we have found that several RPs could be potential CacyBP/SIP targets. Then, by applying various biochemical methods, we confirmed that the interaction between CacyBP/SIP and RPL6 was direct. Moreover, in silico analysis allowed us to establish the domains/fragments of both proteins involved in this interaction. Subsequently, we performed several analyses using neuroblastoma NB2a cell line with stably silenced CacyBP/SIP expression. We have found, by applying OPP (O-propargyl-puromycin), that the number of cells with protein synthesis signal ("translation signal") was significantly lower in the cell line with diminished CacyBP/SIP level than in control one. Moreover, we observed lower induction of the synthesis of Hsp70, a stress-inducible protein, in NB2a cells with diminished CacyBP/SIP level after heat shock. In parallel, we have shown that CacyBP/SIP may have an impact on ER architecture/network. This confirms the influence of CacyBP/SIP, most probably through interaction with RPL6 and/or other RPs, on ribosome function and, in consequence, on protein synthesis in the cell.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Agata Malinowska

LAB HEAD: Ewelina Jurewicz

PROVIDER: PXD064560 | Pride | 2025-07-28

REPOSITORIES: Pride

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CacyBP/SIP - RPL6 interaction: potential influence on ribosome function.

Jurewicz Ewelina E Maksymowicz-Trivedi Małgorzata M Saberi-Khomami Omid O Iwańska Olga O Starosta Agata A Kilańczyk Ewa E Bieganowski Paweł P Jarmuła Adam A Leśniak Wiesława W Filipek Sławomir S Filipek Anna A

Amino acids 20250722 1

Previously, we have shown that CacyBP/SIP interacts with NPM1, a protein involved in ribosome biogenesis. In this work, we extended our previous studies to look for the potential impact of CacyBP/SIP on ribosome biogenesis and/or function. Using mass spectrometry analysis, we have found that several RPs could be potential CacyBP/SIP targets. Since RPL6 was one of the proteins with the best quality scores identified in this analysis we focused on the possible interaction between CacyBP/SIP and RP ...[more]

PMID: 40691326

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			Action	DRS
	208063234maks_IP_FLAG.raw	Raw
	208063235maks_IP_Cacy.raw	Raw
	512172895rosi_Flag.raw	Raw
	512172897rosi_SIP.raw	Raw
	60127111rosi_FLAG.raw	Raw