Dataset Information

NUMB isoforms interactome study

ABSTRACT: HEK293 cells were transfected with NUMB-FLAG isoforms 1,2,3,4. anti-FLAG immunoprecipitationIP of all isoforms plus control sample were loaded on SDS-PAGE gel and CTRL plus ISOform1,2,3,4 lanes upon IP were processed by LC-MSMS analysis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell

SUBMITTER: vittoria matafora

LAB HEAD: Angela Bachi

PROVIDER: PXD064868 | Pride | 2026-06-15

REPOSITORIES: Pride

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Publications

Endocytic Control of Cell-Autonomous and Non-Cell-Autonomous Functions of p53.

Cacciatore Roberta R Basile Andrea A Freddi Stefano S Schiano Lomoriello Irene I Zucca Carlo Ribelle CR Ciossani Giuseppe G Scietti Luigi L Cuomo Alessandro A Ronzoni Simona S Pelicci Simone S Faretta Mario M Zaccheroni Elena E Pelicci Giuliana G Matafora Vittoria V Bachi Angela A Gunby Rosalind Helen RH Pece Salvatore S Sigismund Sara S Lanzetti Letizia L Colaluca Ivan Nicola IN Di Fiore Pier Paolo PP

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20260130 30

NUMB is an endocytic protein with tumor suppressor activity, largely mediated by its ability to inhibit p53 degradation. This function depends on the inclusion of a short alternatively spliced exon (Ex3) in NUMB, although the mechanistic link between endocytosis and p53 regulation remains unclear. Here, we show that the Ex3-encoded sequence directs NUMB to the plasma membrane, where it forms a complex with the endocytic adaptor SNX9. This complex recruits p53 in an SNX9-dependent manner and is i ...[more]

PMID: 41615188

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