Co-Immunoprecipitation of TMEM65

ABSTRACT: Mitochondrial Ca2+ transport is a critical process in cellular physiology. One pathway for export occurs via Na+/Ca2+exchange (mito-NCX) machinery. TMEM65 has recently been shown to be crucial for mito-NCX activity, but its precise mechanistic role remains unknown. To evaluate whether TMEM65 forms a complex with NCLX, a protein previously proposed to facilitate mitochondrial Na+/Ca2+ exchange transport, and to gain insights into TMEM65’s potential functions, we sought to characterize potential interactors of TMEM65, performing co-immunoprecipitation on TMEM65 containing a C-terminal ID4 tag.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293 Cell

SUBMITTER: Shouling Xu  

LAB HEAD: Shou-Ling Xu

PROVIDER: PXD064897 | Pride | 2026-02-09

REPOSITORIES: Pride