Dataset accompanying "Influences of Gaseous Substrate Composition on Single Cell Protein and Poly-3-hydroxybutyrate Production by Cupriavidus necator"

ABSTRACT: Abstract. Microbial gas fermentation remains a promising biotechnology for the production of a variety of industrially relevant chemicals under relatively benign conditions using ubiquitous feedstocks, such as carbon dioxide. Implementation of these gas fermentation processes requires an understanding of the microbial response to different ratios of the gaseous feedstocks (carbon dioxide, oxygen, and hydrogen) supplied to the reactor. Here, we used Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) as a microbial biocatalyst due to its metabolic diversity, genetic tractability, and ability to grow to high cell densities during aerobic fermentation in bioreactors. Specifically, we investigate whether the supply of different proportions of hydrogen and carbon dioxide in the gas stream (1:1, 3:1, and 8:1 H2:CO2) affects the physiology of C. necator, with a focus on the accumulation of single cell protein (SCP) and poly-3-hydroxybutyrate (PHB) within the microbial biomass. We found that an 8:1 ratio of H2:CO2 led to both the highest overall biomass production and PHB content within the biomass after a seven-day incubation. Intracellular SCP content varied as a function of both length of incubation and gas ratio supplied. We conducted a proteomic analysis to determine whether differences in productivity could be correlated with changes in protein expression over time and across treatment groups. This study highlights the importance of considering gaseous substrate composition and its potential effects on microbial physiology and metabolism during fermentation.

INSTRUMENT(S):

ORGANISM(S): Cupriavidus Necator (strain Atcc 17699 / H16 / Dsm 428 / Stanier 337) (ralstonia Eutropha) Bacteria

SUBMITTER: J Hervey  

LAB HEAD: J Hervey

PROVIDER: PXD064900 | Pride | 2026-05-15

REPOSITORIES: Pride