Dataset Information

Novel insights on remnant stomach following Roux-en-Y Gastric Bypass surgery based on histological evaluation and quantitative proteomics analysis

ABSTRACT: Background: Patients undergoing weight-reducing Roux-en-Y gastric bypass (RYGB) have immediate positive effects on metabolic health, including type 2 diabetes (T2D). Refeeding via the secluded stomach, either by a gastrotube or after gastro-gastric fistulation result in T2D relapse and weight regain. The stomach therefore seems to play an active role in metabolism. Objectives: To explore histological and protein expression changes in the gastric mucosa before compared to after RYGB. Methods: Perioperatively, biopsies were taken in a non-paired manner from the stomach (fundus, corpus, antrum) in patients undergoing Sleeve Gastrectomy (SG) and patients >8 months postoperatively after RYGB by balloon-enteroscopy. The included SG and RYGB patients did not display any obvious mucosal or luminal pathology during surgery or the balloon-enteroscopies. The gastric biopsies both at perioperatively and postoperatively were prepared för histological evaluation and for quantitative (comparative) non-targeted proteomics. The results were compared by Volcano plots, Principal Component Analysis and STRING functional protein association networks. Results: Histologically the gastric mucosa looked normal in biopsies from all the different parts of the postoperative bypassed stomach with no clear differences compared to the perioperative samples. The perioperative biopsies generally contained significantly higher amounts of proteins involved in fatty acid metabolism, oxidative phosphorylation and ATP metabolic processes, citric acid cycle and the respiratory chain. Postoperative biopsies instead showed overall increased quantities of proteins associated with ribosomes, RNA-metabolic processes, the mitotic cycle and pancreatic secretion. Conclusions: The results provide novel insights into the mucosal proteome-changes in the secluded stomach following RYGB.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Gastric Mucosa

DISEASE(S): Obesity

SUBMITTER: Evelin Berger

LAB HEAD: Carina Sihlbom Wallem

PROVIDER: PXD065508 | Pride | 2025-07-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	4581_1DSRPeptideProtein_Set3_1mc_3mmu_Antrum_Eclipse_230403_FAIMS_50to85_statistics_sent.xlsx	Xlsx
	4581_1FDRpeptideProtein__Set1_1mc_3mmu_Fundus_Eclipse_230331_FAIMS_4to39_statistics_sent.xlsx	Xlsx
	4581_1FDTprptideProtein_Set2_1mc_3mmu_Corpus_Eclipse_230403_FAIMS_5to40_statistics_sent.xlsx	Xlsx
	4581_Set1_1mc_3mmu_Fundus_Eclipse_230331_FAIMS_4to39.msf	Msf
	4581_Set1_1mc_3mmu_Fundus_Eclipse_230331_FAIMS_4to39.pdResult	Other

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Novel insights on remnant stomach following Roux-en-Y gastric bypass surgery based on histological evaluation and quantitative proteomics analysis.

Larson Carl I W CIW Fändriks Lars L Casselbrant Anna A Wallenius Ville V

Scientific reports 20250712 1

Patients undergoing weight-reducing Roux-en-Y gastric bypass (RYGB) have immediate positive effects on metabolic health, including type 2 diabetes (T2D). Refeeding via the secluded stomach, either by a gastrotube or after gastro-gastric fistulation result in T2D relapse and weight regain. The stomach therefore seems to play an active role in metabolism. To explore histological and protein expression changes in the gastric mucosa before compared to after RYGB. Perioperatively, biopsies were taken ...[more]

PMID: 40652086

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Project description:INTRODUCTION: Bariatric surgery is known to be the most effective treatment for weight loss in obese patients and for the rapid remission of obesity-related comorbidities. These short-term improvements result from not only limited digestion or absorption but also dynamic changes in metabolism throughout the whole body. However, short-term metabolism studies associated with bariatric surgery in Asian individuals have not been reported.OBJECTIVES: The aim of this study was to investigate the short-term metabolome changes in the serum promoted by laparoscopic sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB) and to determine the underlying mechanisms that affect obesity-related comorbidities.METHODS: Serum samples were collected from Korean patients who underwent RYGB or SG before and 4 weeks after the surgery. Metabolomic and lipidomic profiling was performed using UPLC-Orbitrap-MS, and data were analyzed using statistical analysis.RESULTS: Metabolites mainly related to amino acids, lipids (fatty acids, glycerophospholipids, sphingolipids, glycerolipids) and bile acids changed after surgery, and these changes were associated with the lowering of risk factors for obesity-related diseases such as nonalcoholic fatty liver disease (NAFLD), type 2 diabetes (T2D) and atherosclerosis. Interestingly, the number of significantly altered metabolites related to the lipid metabolism were greater in SG than in RYGB. Furthermore, the metabolites related to amino acid metabolism were significantly changed only after SG, whereas bile acid changed significantly only following RYGB.CONCLUSION: These differences could result from anatomical differences between the two surgeries and could be related to the gut microbiota. This study provides crucial information to expand the knowledge of the common but different molecular mechanisms involved in obesity and obesity-related comorbidities affected by each bariatric procedure.